Comparison of loop-mediated isothermal amplification (LAMP) and real-time PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis

文献类型： 外文期刊

第一作者： Lin, Zhibing

作者： Lin, Zhibing;Zhang, Yanlei;Zhang, Houshuang;Zhou, Yongzhi;Cao, Jie;Zhou, Jinlin

作者机构：

关键词： Toxoplasma gondii;Conventional PCR;Loop-mediated isothermal amplification (LAMP);Real-time PCR;529 bp element

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) is a simple method that can amplify DNA with high specificity, sensitivity, and rapidity. In this study, we compared the performance of LAMP and real-time PCR assays for diagnosis of toxoplasmosis. We designed a real-time PCR assay targeting a 529 bp element repeated 200-300 times in the Toxoplasma gondii genome. The detection limits of the LAMP and real-time PCR assays were 10 fg/mu L and 1 fg/mu L of T. gondii DNA, respectively. Conventional PCR, LAMP, and real-time PCR methods were applied to detect T. gondii DNA in blood samples from 284 pigs and 292 sheep. Positive results were obtained with 0.4%, 3.2%, and 4.2% of the pig samples and 3.8%,17.1%, and 17.8% of the sheep samples with conventional PCR, LAMP, and real-time PCR analyses, respectively. The real-time PCR assay provided the most sensitive diagnosis of toxoplasmosis, but the LAMP assay has potential as an alternative tool for detection of T. gondii in the field

分类号： S85

  • 相关文献

[1]The Gene MT3-B Can Differentiate Palm Oil from Other Oil Samples. Zhang, Li,Wu, Gang,Wu, Yuhua,Cao, Yinglong,Xiao, Ling,Lu, Changming.

[2]Comparison of a loop-mediated isothermal amplification for orf virus with quantitative real-time PCR. Wang, Guangxiang,Wang, Yanhua,Liu, Xiangtao,Tian, Hong,Shang, Youjun,Wang, Guangxiang,Shang, Youjun,Wang, Yanhua,Liu, Xiangtao. 2013

[3]Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR. Wang, Yanhua,Wang, Guangxiang,Wang, Meng,Zhang, Delin,Yin, Hong.

[4]Evaluation of a quantitative real-time PCR for rapid detection of Riemerella Anatipestifer infection in birds. Zhang, Qingshan,Li, Chenxi,Bai, Xiaofei,Liu, Ming,Liu, Siguo,Zhang, Yun,Wan, Chunhe. 2017

[5]Loop-mediated isothermal amplification assays for screening of bacterial integrons. Yu, Guangchao,Yu, Guangchao,Li, Bing,Cui, Hemiao,Chen, Siyi,Miao, Jian,Deng, Yang,Chen, Lei,Lin, Chii-wann,Bian, Huawei,Chen, Dingqiang. 2014

[6]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[7]Rapid Detection of Listeria monocytogenes in Raw Milk with Loop-Mediated Isothermal Amplification and Chemosensor. Wang, Deguo,Zhang, Gaiping,Deng, Ruiguang,Zhi, Aimin,Guo, Junqing,Zhao, Dong,Wang, Deguo,Xu, Zhihong,Lu, Chengping. 2011

[8]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[9]Loop-mediated isothermal amplification for detection of porcine circovirus type 2. Han, Si,Shi, Jianli,Wu, Jiaqiang,Yuan, Xiaoyuan,Cong, Xiaoyan,Xu, Shaojian,Wu, Xiaoyan,Li, Jun,Wang, Jinbao,Zhou, Shun,Han, Si,Wu, Xiaoyan,Yuan, Xiaoyuan,Li, Jun,Wang, Jinbao. 2011

[10]Application of an improved loop-mediated isothermal amplification detection of Vibrio parahaemolyticus from various seafood samples. Li, Chunhou,Xu, Zhenbo,Wang, Li,Li, Yue,Zhong, Qingping,Zhao, Xihong,Xu, Zhenbo,Chu, Jin,Li, Yanyan. 2011

[11]Detection of LAMP Products Using CNT Array Electrode. Zhang, Xuzhi,Li, Qiufen,Chen, Bijiuan,Ma, Shaosai,Cui, Yi. 2011

[12]Development of a loop-mediated isothermal amplification assay for rapid detection of iridovirus in the Chinese giant salamander. Meng, Yan,Zhang, Hui,Liang, Hongwei,Zeng, Lingbing,Xiao, Hanbing,Meng, Yan,Xie, Congxin. 2013

[13]Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA. Ren, Weicheng,Cai, Yuyong,Wang, Chongming,Renault, Tristan,Cai, Yuyong. 2010

[14]Development of a loop-mediated isothermal amplification method for rapid detection of porcine boca-like virus. Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Li, Bin,Ma, Jun-jie,Zhang, Xue-han,Wen, Li-bin,Mao, Li,Ni, Yan-xiu,Guo, Rong-li,Zhou, Jun-ming,Lv, Li-xin,He, Kong-wang,Xiao, Shao-bo. 2012

[15]Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification. Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo,Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo. 2012

[16]Rapid and Sensitive Detection of sFAT-1 Transgenic Pigs by Visual Loop-Mediated Isothermal Amplification. Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Yang, Yalan,Li, Xunbi,Li, Kui,Zhou, Rong,Tao, Chenyu,Zhou, Rong,Zheng, Xinmin,Ren, Hongyan.

[17]Reverse transcription loop-mediated isothermal amplification of DNA for detection of Barley yellow dwarf viruses in China. Zhao, Kun,Liu, Yan,Wang, Xifeng. 2010

[18]Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP). Wang, Jianke,Cheng, Shipeng,Yi, Li,Cheng, Yuening,Yang, Shen,Xu, Hongli,Li, Zhenguang,Shi, Xinchuan,Wu, Hua,Yan, Xijun. 2013

[19]Evaluation of loop-mediated isothermal amplification (LAMP) assays based on 5S rDNA-IGS2 regions for detecting Meloidogyne enterolobii. Niu, J. H.,Jian, H.,Guo, Q. X.,Chen, C. L.,Wang, X. Y.,Liu, Q.,Niu, J. H.,Niu, J. H.,Guo, Y. D.. 2012

[20]Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals. Liu, Da-Fei,Chai, Hong-Liang,Yin, Xiu-Chen,Hua, Yu-Ping,Liu, Da-Fei,Liu, Chun-Guo,Tian, Jin,Zhang, Xiao-Zhan,Yang, Tian-Kuo,Zhang, Hong-Ying,Liu, Ming,Qu, Lian-Dong,Jiang, Yi-Tong.

作者其他论文 更多>>

微信小程序