Development of a triplex TaqMan real-time RT-PCR assay for differential detection of wild-type and HCLV vaccine strains of classical swine fever virus and bovine viral diarrhea virus 1

文献类型： 外文期刊

第一作者： Zhang, Xing-Juan

作者： Zhang, Xing-Juan;Han, Qiu-Ying;Sun, Yuan;Zhang, Xin;Qiu, Hua-Ji

作者机构：

关键词： Classical swine fever virus;HCLV;Bovine viral diarrhea virus 1;Triplex real-time RT-PCR

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, genomic sequences of pestiviruses available in GenBank were aligned to design three primer pairs and TaqMan probes: two targeting the NS5A region of the viral genome of classical swine fever virus (CSFV) for the differentiation of wild-type CSFV and hog cholera lapinized vaccine (HCLV) vaccine, and one targeting the 5'-untranslated region of bovine viral diarrhea virus 1 (BVDV-1). With these primers and probes, a triplex TaqMan real-time RT-PCR assay was developed for differentiating wild-type CSFV, the HCLV strain, and BVDV-1. The detection limit of the assay was 4.5 TCID50 for wild-type CSFV, 10 TCID50 for HCLV-strain CSFV, and 3.2 TCID50 for BVDV-1. The triplex real-time RT-PCR had at least 98% (248 samples) agreement with other RT-PCR methods. The assay provides a sensitive tool for simultaneous detection and differentiation of wild-type CSFV and HCLV from BVDV-1. (C) 2011 Elsevier Ltd. All rights reserved.

分类号： S85

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