Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation

文献类型: 外文期刊

第一作者: Tan, Xiuwen

作者: Tan, Xiuwen;Song, Enliang;Liu, Xiaomu;Liu, Guifen;Cheng, Haijian;Wan, Fachun

作者机构:

关键词: Vitrification;Concentration;Supercool X-1000;Ovaries

期刊名称:IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL ( 影响因子:2.416; 五年影响因子:2.117 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The purpose of our study was to investigate the feasibility of using less-concentrated cryoprotectants supplemented with ice blocker Supercool X-1000 to vitrify ovarian tissues. Mouse ovaries were cryopreserved in different concentrations of vitrification solution alone or with Supercool X-1000, and fresh non-frozen ovaries were used as control. The proportions of morphological normality of follicles, normal GCs in follicular fluids and developing to blastocysts were higher in 12.5%ethylene glycol (EG)+12.5% dimethylsulfoxide (DMSO) with Supercool X-1000 than those of treated in 10% EG+10% DMSO or 15% EG+15% DMSO alone or with Supercool X-1000. In conclusion, the inclusion of Supercool X-1000 in less-concentrated vitrification solution was effective to improve the efficiency and efficacy of cryopreservation of ovarian tissues.

分类号: Q95

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