Identification of putative virulence-associated genes of Haemophilus parasuis through suppression subtractive hybridization

文献类型： 外文期刊

第一作者： Zhou, Hongzhuan

作者： Zhou, Hongzhuan;Ma, Rongcai;Zhou, Hongzhuan;Ma, Rongcai;Zhou, Hongzhuan;Yang, Bing;Xu, Fuzhou;Chen, Xiaoling;Wang, Jinluo;Zhang, Peijun;Xia, Yongheng;Zhang, Jin;Blackall, P. J.

作者机构：

关键词： RNA metabolism;genome;stress response;bacterial virulence

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Haemophilus parasuis is the causative agent of Glasser's disease. Up to now 15 serovars of H. parasuis have been identified, with significant differences existing in virulence between serovars. In this study, suppression subtractive hybridization (SSH) was used to identify the genetic difference between Nagasaki (H. parasuis serovar 5 reference strain, highly virulent) and SW114 (H. parasuis serovar 3 reference strain, non-virulent). A total of 191 clones were obtained from the SSH library. Using dot hybridization and PCR, 15 clones were identified containing fragments that were present in the Nagasaki genome while absent in the SW114 genome. Among these 15 fragments, three fragments (ssh1, ssh13, ssh15) encode cell surface-associated components; three fragments (ssh2, ssh5, ssh9) are associated with metabolism and stress response; one fragment (ssh8) is involved in assembly of fimbria and one fragment (ssh6) is a phage phi-105 ORF25-like protein. The remaining seven fragments are hypothetical proteins or unknown. Based on PCR analysis of the 15 serovar reference strains, eight fragments (ssh1, ssh2, ssh3, ssh6, ssh8, ssh10, ssh11 and ssh12) were found in three to five of most virulent serovars (1, 5, 10, 12, 13 and 14), zero to two in three moderately virulent serovars (2,4 and 15), but absent in the low virulent serovar (8) and non-virulent serovars (3, 6, 7, 9 and 11). In vivo transcription fragments ssh1, ssh2, ssh8 and ssh12 were identified in total RNA samples extracted from experimental infected pig lung by RT-PCR. This study has provided some evidence of genetic differences between H. parasuis strains of different virulence

分类号： S8

  • 相关文献

[1]Endosperm-specific hypomethylation, and meiotic inheritance and variation of DNA methylation level and pattern in sorghum (Sorghum bicolor L.) inter-strain hybrids. Zhang, M. S.,Yan, H. Y.,Zhao, N.,Lin, X. Y.,Pang, J. S.,Xu, K. Z.,Liu, L. X.,Liu, B..

[2]The beta-domain of cluster 2b streptokinase is a major determinant for the regulation of its plasminogen activation activity by cellular plasminogen receptors. Zhang, Yueling,Mayfield, Jeffrey A.,Ploplis, Victoria A.,Castellino, Francis J.,Zhang, Yueling,Mayfield, Jeffrey A.,Ploplis, Victoria A.,Castellino, Francis J.,Zhang, Yueling. 2014

[3]Trigger factor of Streptococcus suis is involved in stress tolerance and virulence. Wu, Tao,Ma, Hongwei,Lu, Ka,Ren, Wen,Liu, Zhengya,Qiu, Yinsheng,Chen, Huanchun,Wu, Tao,Zhao, Zhanqin,Chang, Haitao,Bei, Weicheng,Chen, Huanchun,Zhang, Lin,Zhao, Zhanqin.

[4]Burkholderia cenocepacia integrates cis-2-dodecenoic acid and cyclic dimeric guanosine monophosphate signals to control virulence. Yang, Chunxi,Cui, Chaoyu,Ye, Qiumian,Fu, Shuna,Song, Shihao,Huang, Yutong,Zhang, Lian-Hui,Deng, Yinyue,Yang, Chunxi,Cui, Chaoyu,Ye, Qiumian,Fu, Shuna,Song, Shihao,Huang, Yutong,Harwood, Caroline S.,Deng, Yinyue,Yang, Chunxi,Cui, Chaoyu,He, Fei,Zhang, Lian-Hui,Deng, Yinyue,Yang, Chunxi,Gao, Yong-Gui,Kan, Jinhong,Jia, Yantao,Harwood, Caroline S.. 2017

[5]Complete Genome Sequences of Brucella melitensis Strains M28 and M5-90, with Different Virulence Backgrounds. Bu, Zhigao.

[6]A wheat PI4K gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in Arabidopsis. Liu, Pei,Xu, Zhao-Shi,Lu, Pan-Pan,Hu, Di,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2013

[7]The effect of stocking density on growth and seven physiological parameters with assessment of their potential as stress response indicators for the Atlantic salmon (Salmo salar). Liu, Baoliang,Liu, Ying,Liu, Baoliang,Wang, Xianping. 2015

[8]TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis. Mao, Xinguo,Zhang, Hongying,Qian, Xueya,Li, Ang,Zhao, Guangyao,Jing, Ruilian.

[9]Transgenic expression of TaMYB2A confers enhanced tolerance to multiple abiotic stresses in Arabidopsis. Mao, Xinguo,Jia, Dongsheng,Li, Ang,Zhang, Hongying,Tian, Shanjun,Jia, Jizeng,Jing, Ruilian,Mao, Xinguo,Jia, Dongsheng,Li, Ang,Zhang, Hongying,Tian, Shanjun,Jia, Jizeng,Jing, Ruilian,Jia, Dongsheng,Zhang, Hongying,Zhang, Xiaoke.

[10]Identification of ERF genes in peanuts and functional analysis of AhERF008 and AhERF019 in abiotic stress response. Wan, Liyun,Wu, Yanshan,Huang, Jiaquan,Lei, Yong,Yan, Liying,Jiang, Huifang,Liao, Boshou,Dai, Xiaofeng,Zhang, Juncheng,Varshney, Rajeev K..

[11]A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses. Zheng Jingyuan,Mao Zhenchuan,Xie Bingyan,Zheng Jingyuan,Zou Xuexiao.

[12]Effects of repeated handling and air exposure on the immune response and the disease resistance of gibel carp (Carassius auratus gibelio) over winter. Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Tu, Yongqin,Hu, Huihua,Wang, Cuicui,Han, Dong.

[13]Isolation and characterization of a novel EAR-motif-containing gene GmERF4 from soybean (Glycine max L.). Zhang, Gaiyun,Chen, Xueping,Guo, Jiaming,Chen, Ming,Xu, Zhaoshi,Li, Liancheng,Ma, Youzhi.

[14]Molecular cloning and functional analysis of the drought tolerance gene MsHSP70 from alfalfa (Medicago sativa L.). Li, Zhenyi,Long, Ruicai,Zhang, Tiejun,Wang, Zhen,Zhang, Fan,Yang, Qingchuan,Kang, Junmei,Sun, Yan.

[15]Functions of the ERF transcription factor family in plants. Xu, Zhao-Shi,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi.

[16]Genome-Wide Analysis of the C3H Zinc Finger Transcription Factor Family and Drought Responses of Members in Aegilops tauschii. Jiang, An-Long,Xu, Zhao-Shi,Zhao, Guang-Yao,Cui, Xiao-Yu,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi.

[17]Functional identification of apple MdJAZ2 in Arabidopsis with reduced JA-sensitivity and increased stress tolerance. An, Xiu-Hong,Li, En-Mao,Xu, Kai,Cheng, Cun-Gang,Hao, Yu-Jin.

[18]Genome-wide identification, expression profiling, and SSR marker development of the bZIP transcription factor family in Medicago truncatula. Zhang, Zhengshe,Liu, Wenxian,Liu, Zhipeng,Xie, Wengang,Wang, Yanrong,Qi, Xiao,Qi, Xiao.

[19]Comparative Transcriptional Profiling of Melatonin Synthesis and Catabolic Genes Indicates the Possible Role of Melatonin in Developmental and Stress Responses in Rice. Wei, Yunxie,Zeng, Hongqiu,He, Chaozu,Shi, Haitao,Hu, Wei,Chen, Lanzhen. 2016

[20]Roles of the bZIP gene family in rice. E, Z. G.,Zhang, Y. P.,Wang, L.,Wang, L.,Zhou, J. H.,Zhou, J. H.. 2014

作者其他论文 更多>>

微信小程序