Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity
文献类型: 外文期刊
第一作者: Chen, Tingting
作者: Chen, Tingting;Sun, Yan;Chen, Tingting;Yang, Qingchuan;Kang, Junmei;Ding, Wang;Zhang, Tiejun;Chen, Tingting;Zhang, Xinquan;Gruber, Margaret
作者机构:
关键词: Alfalfa;Ethylene response factor gene;Expression analysis;Subcellular localization;Transgenic tobacco;Salt stress
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
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年卷期:
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收录情况: SCI
摘要: Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized from alfalfa. The MsERF8 cDNA has an open reading frame of 603 bp and encodes a nuclear protein of 201 amino acids. Q-RT-PCR analysis revealed that MsERF8 was strongly enriched in roots and leaves compared with stems, flower buds and flowers of mature alfalfa plants. Bioinformatic analysis of the MsERF8 promoter indicated a number of elements associated with stress-related responses, and MsERF8 transcripts in alfalfa seedlings were induced by NaCl, PEG6000, Al-2(SO4)(3) and five different hormones. Expression of MsERF8 in transgenic tobacco plants resulted in higher tolerance to salinity than with non-transgenic plants. This data shows that MsERF8 is a gene which prevents or alleviates salinity damage and has strong potential to impart salt tolerance to other crop plants.
分类号: Q7
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