Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus

文献类型： 外文期刊

第一作者： Yan, Liping

作者： Yan, Liping;Peng, Shan;Yan, Pixi;Zhou, Jiewen;Teng, Qiaoyang;Li, Guoxin;Li, Xuesong;Li, Zejun;Li, Zejun

作者机构：

关键词： Reverse transcription loop-mediated isothermal amplification;Duck Tembusu virus;Real-time reverse transcription polymerase chain reaction;Comparison

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Duck Tembusu virus (DTMUV) has caused huge losses to the poultry industry in China since the spring of 2010. The development of a rapid, convenient, and reliable method to diagnose this emerging duck infectious disease is critical. In the present study, a real-time reverse transcription loop-mediated isothermal amplification (RI-LAMP) assay was compared with the real-time reverse transcription polymerase chain reaction (RT-PCR) for detection of DTMUV. The sensitivity of real-time RT-LAMP was equal to that of the real-time RT-PCR, with a detection limit of 0.01 ELD50 (50% egg lethal dose). The specificity of the real-time RT-LAMP and real-time RT-PCR was confirmed using RNAs and DNAs extracted from related viruses which cause duck infections. The reproducibility of the real-time RT-PCR assay was better than that of the real-time RT-LAMP. Only three results from 96 tissue samples differed between the real-time RI-LAMP and this real-time RT-PCR, confirming the reliability of these methods. This study indicated that the real-time RI-LAMP is simpler, less time-consuming, and more convenient than the real-time RT-PCR. With its high sensitivity, specificity, and convenience, the real-time RT-LAMP is a practical molecular diagnostic method for rapid and quantitative detection of DTMUV infection in a resource-limited setting. (C) 2012 Elsevier B.V

分类号： R37

  • 相关文献

[1]Rapid typing of foot-and-mouth disease serotype Asia 1 by reverse transcription loop-mediated isothermal amplification. Liu, Yong-sheng,Liu, Xiang-tao. 2011

[2]A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C. Ding, Yao-zhong,Zhou, Jian-hua,Ma, Li-na,Qi, Yan-ni,Zhang, Jie,Zhang, Yong-guang,Ding, Yao-zhong,Zhang, Jie,Zhang, Yong-guang,Wei, Gang.

[3]Rapid detection of Betacoronavirus 1 from clinical fecal specimens by a novel reverse transcription loop-mediated isothermal amplification assay. Qiao, Jun,Meng, Qingling,Chen, Chuangfu,Zhang, Zaichao,Tian, Zhenzhong,Cai, Xuepeng. 2012

[4]Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Liu, Yong-sheng,Liu, Xiang-tao. 2011

[5]Development of a reverse transcription loop-mediated isothermal amplification assay for detection of Porcine teschovirus. Wang, Bin,Tong, Guang-Zhi,Wang, Bin,Wang, Yongqiang,Tian, Zhi-Jun,An, Tong-Qing,Peng, Jin-Mei,Wang, Bin,Peng, Jin-Mei,Tong, Guang-Zhi. 2011

[6]Reverse transcription loop-mediated isothermal amplification for rapid and quantitative assay of covert mortality nodavirus in shrimp. Zhang, Qingli,Liu, Shuang,Yang, Haolin,Zhu, Luoluo,Wan, Xiaoyuan,Li, Xiaoping,Huang, Jie,Zhang, Qingli,Wan, Xiaoyuan,Huang, Jie,Zhang, Qingli,Liu, Shuang,Yang, Haolin,Zhu, Luoluo,Li, Xiaoping,Huang, Jie.

[7]Reverse transcription loop-mediated isothermal amplification assay for rapid detection of Papaya ringspot virus. Zhou, Peng.

[8]Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus. Yun, Tao,Ni, Zheng,Hua, Jionggang,Ye, Weicheng,Chen, Liu,Zhang, Shuai,Zhang, Yan,Zhang, Cun,Zhang, Shuai. 2012

[9]The vaccine efficacy of recombinant duck enteritis virus expressing secreted E with or without PrM proteins of duck tembusu virus. Chen, Pucheng,Liu, Jinxiong,Jiang, Yongping,Zhao, Yuhui,Li, Qimeng,Wu, Li,He, Xijun,Chen, Hualan.

[10]Development of a solid-phase competition ELISA to detect antibodies against newly emerged Duck Tembusu virus. Fu, Yuguang,Ji, Yanhong,Liu, Bin,Dafallah, Rihab Muhammad,Zhu, Qiyun.

[11]Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein. Liu, Y..

[12]Development of a live attenuated vaccine candidate against duck Tembusu viral disease. Li, Guoxin,Gao, Xuyuan,Xiao, Yali,Liu, Shaoqiong,Peng, Shan,Li, Xuesong,Shi, Ying,Zhang, Yuee,Yu, Lei,Wu, Xiaogang,Yan, Pixi,Yan, Liping,Teng, Qiaoyang,Tong, Guangzhi,Li, Zejun. 2014

[13]An adapted duck Tembusu virus induces systemic infection and mediates antibody-dependent disease severity in mice. Liu, Zongliang,Ji, Yanhong,Huang, Xiaohui,Fu, Yuguang,Cai, Xuepeng,Zhu, Qiyun,Wei, Jianzhong.

[14]Development and Validation of One-Step SYBR Green Real-Time RT-PCR for the Rapid Detection of Newly Emerged Duck Tembusu Virus. Liu, Zongliang,Fu, Yuguang,Ji, Yanhong,Cai, Xuepeng,Zhu, Qiyun,Wei, Jianzhong. 2013

[15]Duck tembusu virus and its envelope protein induce programmed cell death. Shaozhou, Wulin,Li, Chenxi,Zhang, Qingshan,Meng, Runzhe,Gao, Youlan,Liu, Hongyu,Bai, Xiaofei,Chen, Yuhuan,Liu, Ming,Liu, Siguo,Zhang, Yun.

[16]Epitope Identification and Application for Diagnosis of Duck Tembusu Virus Infections in Ducks. Li, Chenxi,Shaozhou, Wulin,Bai, Xiaofei,Zhang, Qingshan,Hua, Ronghong,Liu, Ming,Zhang, Yun,Liu, Junyan,Liu, Jyung-Hurng. 2016

[17]Efficacy Evaluation of an Inactivated Duck Tembusu Virus Vaccine. Lin, Jian,Liu, Yuehuan,Yang, Zhiyuan,Duan, Huijuan,Xie, Jia,Zhao, Jicheng,Pan, Jie,Wang, Xiuqing,Yang, Baoshou,He, Pingyou,Zou, Lihong.

[18]Genomic analysis of an effective lentiviral vaccine-attenuated equine infectious anemia virus vaccine EIAV(FDDV13). Qi, Xu,Su, Zhiqiang,Wang, Xuefeng,Wang, Shuai,Lin, Yuezhi,Jiang, Chenggang,Ma, Jian,Zhao, Liping,Lv, Xiaoling,Shen, Rongxian,Kong, Xiangang,Zhou, Jianhua,Wang, Xuefeng,Wang, Fenglong,Ma, Jian. 2010

[19]Comparison of the Effect of Sterilization Pretreatment Using Four Different Bacteriostatic Agents on the Quality of Penaeus Vannamei. Wei, Ya,Zhao, Yongqiang,Hao, Shuxian,Cen, Jianwei,Huang, Hui,Lin, Wanling,Yang, Xianqing. 2014

[20]Comparison of three common DNA concentration measurement methods. Li, Xiaofei,Wu, Yuhua,Zhang, Li,Cao, Yinglong,Li, Yunjing,Li, Jun,Zhu, Li,Wu, Gang.

作者其他论文 更多>>

微信小程序