Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production

文献类型： 外文期刊

第一作者： Lee, Learn-Han

作者： Lee, Learn-Han;Cheah, Yoke-Kqueen;Ab Mutalib, Nurul-Syakima;Sidik, Shiran Mohd;Tang, Yi-Li;Lin, Hai-Peng;Hong, Kui;Hong, Kui

作者机构：

关键词： Diversity;Actinobacteria;High-throughput screening;16S rRNA;ERIC-PCR;RAPD

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The present study aimed to isolate actinobacteria from soil samples and characterized them using molecular tools and screened their secondary metabolites for antimicrobial activities. Thirty-nine strains from four different location of Barrientos Island, Antarctica using 12 types of isolation media was isolated. The isolates were preceded to screening of secondary metabolites for antimicrobial and antifungal activities. Using high-throughput screening methods, 38% (15/39) of isolates produced bioactive metabolites. Approximately 18% (7/39), 18% (7/39), 10% (4/39) and 2.5% (1/39) of isolates inhibited growth of Candida albicans ATCC 10231(T), Staphylococcus aurues ATCC 51650(T), methicillin-resistant Staphylococcus aurues (MRSA) ATCC BAA-44(T) and Pseudomonas aeruginosa ATCC 10145(T), respectively. Molecular characterization techniques like 16S rRNA analysis, Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), Random amplified polymorphic DNA (RAPD) and composite analyses were used to characterize the actinobacteria strains. Analysis of 16S rRNA sequences is still one of the most powerful methods to determine higher taxonomic relationships of Actinobacteria. Both RAPD and ERIC-PCR fingerprinting have shown good discriminatory capability but RAPD proved to be better in discriminatory power than ERIC-PCR. Our results demonstrated that composite analysis of both fingerprinting generally increased the discrimination ability and generated best clustering for actinobacteria strains in this study.

分类号： Q93

  • 相关文献

[1]Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites. Lee, L. -H.,Cheah, Y. -K.,Syakima, A. M. Nurul,Shiran, M. S.,Tang, Y. -L.,Lin, H. -P.,Hong, K.,Hong, K.. 2012

[2]Culturable actinobacteria isolated from marine sponge Iotrochota sp.. Jiang, Shumei,Li, Xiang,Sun, Wei,Dai, Shikun,Xie, Lianwu,Zhang, Long,Liu, Yonghong,Lee, Kyung Jin.

[3]Random amplified polymorphism of DNA analysis in Aegilops tauschii. Kong, LR,Dong, YC,Jia, JZ. 1998

[4]Diversity of degradation pathways of some aromatic compounds by phenotype and genotype testing in Acinetobacter strains. Yu, XQ,Belhaj, A,Elmerich, C,Lin, M. 2004

[5]Micromonospora vulcania sp nov., isolated from volcanic sediment. Liu, Chongxi,Zhou, Shuyu,Li, Jiansong,Shen, Yue,Guan, Xuejiao,Guo, Siyu,Gao, Meiyue,Wang, Xiangjing,Xiang, Wensheng.

[6]Identification and Efficiency of a Nitrogen-fixing Endophytic Actinobacterial Strain from Sugarcane. Pang, Fei,Singh, Rajesh Kumar,Yang, Li-Tao,Li, Hai-Bi,Zhu, Kai,Xing, Yong-Xiu,Solanki, Manoj Kumar,Li, Yang-Rui. 2017

[7]Haloactinobacterium album gen. nov., sp. nov., a halophilic actinobacterium, and proposal of Ruaniaceae fam. nov.. Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Tang, Shu-Kun,Zhi, Xiao-Yang,Wu, Jin-Yuan,Xu, Li-Hua,Li, Wen-Jun,Wang, Yun,Lou, Kai,Lee, Jae-Chan,Kim, Chang-Jin.

[8]Actinomycetes for Marine Drug Discovery Isolated from Mangrove Soils and Plants in China. Hong, Kui,Xie, Qing-Yi,Zhuang, Ling,Lin, Hai-Peng,Ruan, Ji-Sheng,Gao, An-Hui,Yu, Hai-Ping,Li, Jia,Gao, Hao,Yao, Xin-Sheng,Goodfellow, Michael,Ruan, Ji-Sheng. 2009

[9]Applications of Replicating-Competent Reporter-Expressing Viruses in Diagnostic and Molecular Virology. Li, Yongfeng,Li, Lian-Feng,Yu, Shaoxiong,Wang, Xiao,Zhang, Lingkai,Yu, Jiahui,Xie, Libao,Qiu, Hua-Ji,Li, Weike,Ali, Razim. 2016

[10]On-chip investigation of cell-drug interactions. Zheng, Xin Ting,Yu, Ling,Li, Chang Ming,Zheng, Xin Ting,Li, Chang Ming,Li, Peiwu,Li, Peiwu,Dong, Hua,Wang, Yingjun,Dong, Hua,Wang, Yingjun,Liu, Yun.

[11]A high-throughput screening method for identifying lycopene-overproducing E. coli strain based on an antioxidant capacity assay. Xu, Xian,Jin, Weiyue,Xu, Qing,Li, Shuang,Huang, He,Jiang, Ling,Zhang, Zhidong,Huang, He.

[12]Bioactivities of six sterols isolated from marine invertebrates. Zhou, Xuefeng,Ma, Wanlei,Chen, Zhefan,Yang, Bin,Liu, Yonghong,Sun, Jianfan,Fang, Wei.

[13]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[14]Amplification of 16S rRNA genes of Anaplasma species in China for phylogenetic analysis. Liu, ZJ,Luo, JX,Bai, Q,Ma, ML,Guan, GG,Yin, H. 2005

[15]An outbreak of granulomatous inflammation associated with Francisella noatunensis subsp orientalis in farmed tilapia (Oreochromis niloticus x O-aureus) in China. Lin Qiang,Li Ningqiu,Fu Xiaozhe,Hu Qiandong,Chang Ouqin,Liu Lihui,Zhang Defeng,Wang Guangjun,Wu Shuqin,Lin Qiang,Li Ningqiu,Fu Xiaozhe,Liu Lihui,Zhang Defeng,Wu Shuqin,San Guibao. 2016

[16]Isolation and identification of multidrug-resistant Staphylococcus haemolyticus from a laboratory-breeding mouse. Huang, Fengying,Meng, Qiuping,Tan, Guanghong,Huang, Yonghao,Wang, Hua,Huang, Fengying,Huang, Fengying,Mei, Wenli,Dai, Haofu. 2011

[17]Identification of a Gram Negative Rahnella aquatilis Strain from Rana temporaria chensinensis David in China. Xue, Yuan,Zhang, Duanling,Meng, Yang,Zhang, Yanlong,Chen, Jianfei. 2013

[18]Three new species of the genus Actinobispora of the family Pseudonocardiaceae, Actinobispora alaniniphila sp. nov., Actinobispora aurantiaca sp. nov. and Actinobispora xinjiangensis sp. nov.. Xu, LH,Jin, X,Mao, PH,Lu, ZF,Cui, XL,Jiang, CL. 1999

[19]Pseudonocardia sichuanensis sp nov., a novel endophytic actinomycete isolated from the root of Jatropha curcas L.. Qin, Sheng,Xing, Ke,Cao, Cheng-Liang,Sun, Yong,Jiang, Ji-Hong,Li, Wen-Jun,Li, Wen-Jun,Fei, Shi-Min,Lin, Qiang,Chen, Xiu-Min,Wang, Yun. 2011

[20]Diversity of bacterioplankton in the surface seawaters of Drake Passage near the Chinese Antarctic station. Xing, Mengxin,Li, Zhao,Wang, Wei,Sun, Mi. 2015

作者其他论文 更多>>

微信小程序