Characterization of thioredoxin glutathione reductase in Schiotosoma japonicum

文献类型： 外文期刊

第一作者： Han, Yanhui

作者： Han, Yanhui;Zhang, Min;Hong, Yang;Zhu, Zhu;Li, Dong;Fu, Zhiqiang;Lin, Jiaojiao;Han, Yanhui;Li, Xiangrui

作者机构：

关键词： Schistosoma japonicum;Thioredoxin glutathione reductase;Clone;Vaccine

期刊名称：PARASITOLOGY INTERNATIONAL （ 影响因子：2.23； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： Schistosomiasis is one of the most prevalent and serious parasitic diseases in the world and remains an important public health problem in China. Screening and discovery of an effective vaccine candidate or new drug target is crucial for the control of this disease. In this study, we cloned a cDNA encoding Schistosoma japonicum (S. japonicum) thioredoxin glutathione reductase (SjTGR) from the cDNA of 42-day-old adult worms. The open reading frame (ORF) of the gene was 1791 base pairs (bp) encoding aprotein of 596 amino acids. SjTGR was subcloned into pET-32a ( + ) and expressed in Escherichia coli (E. coli) BL21 (DE3). The recombinant protein rSjTGR exhibited enzymatic activity of 5.13 U/mg with DTNB as the substrate, and showed strong immunogenecity. Real-time PCR results indicated that SjTGR was expressed at a higher level in 35-day-old schistosome worms in transcript. We vaccinated BALB/c mice with rSjTGR in combination with MONTANIDE? ISA 206 VG (ISA 206) and observed a 33.50% to 36.51% (P<0.01) decrease in the adult worm burden and a 33.73%to 43.44% (P<0.01) decrease in the number of eggs counted compared to the ISA 206 or blank control groups in two independent vaccination tests. ELISA analysis demonstrated that rSjTGR induced a high levelof SjTGR-speciflc IgG, IgGl, and IgG 2a antibodies and induced elevated production of IFN-gamma. This study provides the basis for further investigations into the biological function of SjTGR and further evaluation of the potential use of this molecule as a vaccine candidate or new drug target is warranted.

分类号： Q18

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