Selective hydrolysis by commercially available hesperidinase for isoquercitrin production

文献类型： 外文期刊

第一作者： Wang, Jun

作者： Wang, Jun;Ma, Yan-Long;Xia, Rui;Sun, Guo-Xia;Wu, Fu-An;Wang, Jun;Wu, Xiang-Yang;Yu, Liang;Wu, Fu-An;Yu, Liang

作者机构：

关键词： Hesperidinase;α-L-Rhamnosidase;Isoquercitrin;Rutin;Biocatalysis

期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Isoquercitrin is a rare flavonol glycoside with various biological activities, and it is a primary synthetic precursor for enzymatically modified isoquercitrin (EMIQ), which was recently approved as a multiple food additive. The enzymatic method has a great potential to produce isoquercitrin from hydrolysis of rutin via selectively removing a terminal rhamnose. Commercially available hesperidinase was investigated and found that α-L-rhamnosidase achieves good catalytic capacity while β-D-glucosidase loses its activity at pH 7.0. The increased conversion of rutin to isoquercitrin was found by the addition of some metal ions (K~+, Li~+, Mg~(2+), Zn~(2+) and Al~(3+)). Electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) were used to identify isoquercitrin from the transformed products. The enzymatic reaction conditions (temperature, substrate concentration, and reaction time) of isoquercitrin from rutin were optimized, and the apparent kinetic parameters V_m/K_m at different temperatures were also measured. The results suggest that the commercially available hesperidinase can be applied into isoquercitrin production via highly selective hydrolysis.

分类号： O64

  • 相关文献

[1]Enhancement of the selective enzymatic biotransformation of rutin to isoquercitrin using an ionic liquid as a co-solvent. Wang, Jun,Sun, Guo-Xia,Wu, Fu-An,Guo, Xi-Jie,Yu, Liang,Wu, Fu-An,Guo, Xi-Jie,Yu, Liang.

[2]A comparison of acidic and enzymatic hydrolysis of rutin. Wang, Jun,Zhao, Lu-Lu,Sun, Guo-Xia,Liang, Yao,Wu, Fu-An,Wu, Fu-An,Chen, Zhong-li,Cui, Shi-ming,Chen, Zhong-li,Cui, Shi-ming. 2011

[3]Enhanced biocatalysis mechanism under microwave irradiation in isoquercitrin production revealed by circular dichroism and surface plasmon resonance spectroscopy. Gong, An,Zhu, Dan,Mei, Yi-Yuan,Xu, Xiao-Hui,Wu, Fu-An,Wang, Jun,Gong, An,Zhu, Dan,Mei, Yi-Yuan,Xu, Xiao-Hui,Wu, Fu-An,Wang, Jun,Wu, Fu-An,Wang, Jun.

[4]A peculiar segmented flow microfluidics for isoquercitrin biosynthesis based on coupling of reaction and separation. Gong, An,Gu, Shuang-Shuang,Wang, Jun,Sheng, Sheng,Wu, Fu-An,Gong, An,Gu, Shuang-Shuang,Wang, Jun,Sheng, Sheng,Wu, Fu-An,Wang, Jun,Sheng, Sheng,Wu, Fu-An.

[5]Isoquercitrin production from rutin catalyzed by naringinase under ultrasound irradiation. Zhu, Dan,Gong, An,Xu, Yan,Tsabing, D'assise Kinfack,Wu, Fuan,Wang, Jun,Wu, Fuan,Wang, Jun.

[6]Application of near-infrared reflectance spectroscopy to the evaluation of rutin and D-chiro-inositol contents in tartary buckwheat. Yang, Nan,Ren, Guixing.

[7]Effect of dietary supplementation of rutin on lactation performance, ruminal fermentation and metabolism in dairy cows. Cui, K.,Guo, X. D.,Tu, Y.,Zhang, N. F.,Ma, T.,Diao, Q. Y..

[8]FtSAD2 and FtJAZ1 regulate activity of the FtMYB11 transcription repressor of the phenylpropanoid pathway in Fagopyrum tataricum. Zhou, Meiliang,Sun, Zhanmin,Ding, Mengqi,Wang, Dan,Tang, Yixiong,Wu, Yanmin,Zhou, Meiliang,Zhu, Xuemei,Logacheva, Maria D.,Kreft, Ivan,Yan, Mingli,Shao, Jirong. 2017

[9]Bioactive compounds in functional buckwheat food. Shao, Ji-Rong,Zhang, Zhan-Lu,Zhou, Mei-Liang,Tang, Yi-Xiong,Shao, Ji-Rong,Zhang, Zhan-Lu,Xue, Wen-Tong,Zhang, Zhan-Lu,Zhou, Mei-Liang,Wu, Yan-Min,Tang, Yu,Li, Fa-Liang.

[10]The Effect of Administration of Rutin on Plasma Levels of Estrogen, Prolactin, Growth Hormone and Gene Expression of Their Receptors in Mammary Glands in Ovariectomized Rats. Guo Xu-dong,Diao Qi-yu,Tu Yan,Wang Yue-ying,Wang Xin-jian,Fu Tong,Deng Kai-dong,Yan Gui-long. 2012

[11]Influence of Rhizoglomus irregulare on nutraceutical quality and regeneration of Lycium barbarum leaves under salt stress. Liu, Hongguang,Liu, Hongguang,Wang, Yajun,Chen, Hui,Tang, Ming.

[12]Enantio-selective preparation of (S)-1-phenylethanol by a novel marine GDSL lipase MT6 with reverse stereo-selectivity. Deng, Dun,Zhang, Yun,Sun, Aijun,Hu, Yunfeng,Deng, Dun,Zhang, Yun,Sun, Aijun,Hu, Yunfeng,Deng, Dun,Hu, Yunfeng. 2016

[13]Production of Adipic Acid from Sugar Beet Residue by Combined Biological and Chemical Catalysis. Zhang, Hongfang,Su, Xiaoyun,Ang, Ee Lui,Zhao, Huimin,Li, Xiukai,Zhang, Yugen,Su, Xiaoyun,Zhao, Huimin,Zhao, Huimin,Zhao, Huimin. 2016

[14]Chemical structures, biosynthesis, bioactivities, biocatalysis and semisynthesis of tobacco cembranoids: An overview. Yan, Ning,Du, Yongmei,Liu, Xinmin,Zhang, Hongbo,Liu, Yanhua,Zhang, Peng,Gong, Daping,Zhang, Zhongfeng.

[15]Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition. Zheng, Ming-Ming,Huang, Feng-Hong,Wang, Lian,Guo, Ping-Mei,Deng, Qian-Chun,Zheng, Ming-Ming,Huang, Feng-Hong,Wang, Lian,Guo, Ping-Mei,Deng, Qian-Chun,Lu, Yong,Feng, Yu-Qi.

[16]Structured lipids enriched with unsaturated fatty acids produced by enzymatic acidolysis of silkworm pupae oil using oleic acid. Zhao, Xing-Yu,Wang, Xu-Dong,Liu, Xi,Zhu, Wei-Jie,Mei, Yi-Yuan,Li, Wen-Wen,Wang, Jun,Wang, Jun.

[17]Immobilization of Candida rugosa lipase on hydrophobic/strong cation-exchange functional silica particles for biocatalytic synthesis of phytosterol esters. Zheng, Ming-Ming,Dong, Ling,Guo, Ping-Mei,Deng, Qian-Chun,Li, Wen-Lin,Huang, Feng-Hong,Lu, Yong,Feng, Yu-Qi.

[18]A novel chemoenzymatic synthesis of propyl caffeate using lipase-catalyzed transesterification in ionic liquid. Pang, Na,Gu, Shuang-Shuang,Wang, Jun,Cui, Hong-Sheng,Wang, Fang-Qin,Liu, Xi,Zhao, Xing-Yu,Wu, Fu-An,Wu, Fu-An.

[19]Cooperative Reinforcement of Ionic Liquid and Reactive Solvent on Enzymatic Synthesis of Caffeic Acid Phenethyl Ester as an In Vitro Inhibitor of Plant Pathogenic Bacteria. Xu, Yan,Sheng, Sheng,Liu, Xi,Wang, Chao,Xiao, Wei,Wang, Jun,Wu, Fu-An,Xu, Yan,Sheng, Sheng,Wang, Jun,Wu, Fu-An.

[20]Enhancement of Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquid with DMSO Co-solvent. Gu, Shuangshuang,Wang, Jun,Wei, Xianbin,Cui, Hongsheng,Wu, Xiangyang,Wu, Fuan,Wang, Jun,Wang, Jun,Wu, Fuan. 2014

作者其他论文 更多>>

微信小程序