Functional characterizations of malonyl-CoA:acyl carrier protein transacylase (MCAT) in Eimeria tenella

文献类型： 外文期刊

第一作者： Zhu, Guan

作者： Zhu, Guan;Sun, Mingfei;Qin, Zonghua;Wu, Caiyan;Lv, Minna;Liao, Shenquan;Qi, Nanshan;Xie, Mingquan;Cai, Jianping;Sun, Mingfei;Qin, Zonghua;Wu, Caiyan;Lv, Minna;Liao, Shenquan;Qi, Nanshan;Xie, Mingquan;Cai, Jianping

作者机构：

关键词： Coccidiosis;Corytuberine;Eimeria tenella;Malonyl-CoA:acyl-carry protein transacylase;Type II fatty acid synthase

期刊名称：MOLECULAR AND BIOCHEMICAL PARASITOLOGY （ 影响因子：1.759； 五年影响因子：2.135 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Eimeria tenella, an apicomplexan parasite in chickens, possesses an apicoplast and its associated metabolic pathways including the Type II fatty acid synthesis (FAS II). Malonyl-CoA:acyl-carry protein transacylase (MCAT) encoded by the fabD gene is one of the essential enzymes in the FAS II system. In the present study, the entire E. tenella MCAT gene (EtfabD) was cloned and sequenced. Immunolabeling located this protein in the apicoplast organelle in coccidial sporozoites. Functional replacement of the fabD gene with amber mutation of E. coli temperature-sensitive LA2-89 strain by E. tenella EtMCAT demonstrated that EcFabD and EtMCAT perform the same biochemical function. The recombinant EtMCAT protein was expressed and its general biochemical features were also determined. An alkaloid natural product corytuberine (CAS: 517-56-6) could specifically inhibit the EtMCAT activity (IC 50 = 16.47 μM), but the inhibition of parasite growth in vitro by corytuberine was very weak (the predicted MIC 50 = 0.65 mM).

分类号： R38

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