Lowering the Blood Glucose of Diabetes Mellitus Mice by Oral Administration with Transgenic Human Insulin-like Growth Factor I Silkworms

文献类型： 外文期刊

第一作者： Xue, Renyu

作者： Xue, Renyu;Wang, Yang;Cao, Guangli;Pan, Zhonghua;Zheng, Xiaojian;Gong, Chengliang;Xue, Renyu;Cao, Guangli;Pan, Zhonghua;Gong, Chengliang;Zhou, Wenlin

作者机构：

关键词： transgenic silkworm;hlGF-I;DM mice;reducing blood glucose

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To evaluate the biological activity of the posterior silk glands of transgenic silkworms expressing human insulinlike growth factor I (hIGF-l), we bred hIGF-I-transgenic silkworms through eight generations by continuously selecting with green fluorescence and G418. The G8 transgenic silkworms were confirmed by polymerase chain reaction and dot blotting, and their posterior silk glands were removed from the fifth instar larvae to make freeze-dried powders. Enzyme-linked immunosorbent assay results showed that the expression level of hIGF-I in the posterior silk glands of G8 transgenic silkworm is approximately 493 ng/g of freeze-dried powder. When the freeze-dried powder was administrated by gavage to diabetes mellitus (DM) mice, the blood glucose in DM mice significantly decreased (P < 0.05) in a time- and dose-dependent manner compared with that of DM mice orally administrated with distilled water and normal freeze-dried powders made of untreated silk glands. These results demonstrated that hIGF-I expressed in posterior silk glands of transgenic silkworms could reduce blood glucose by oral administration.

分类号： R15

  • 相关文献

[1]Transformation of neomycin resistance gene (neo(R)) into silkworm (Bombyx mori L.). Chen, X,Zhao, Y,Zhang, F,Peng, WP,Feng, XL,Huang, JT,Lu, CD. 1999

[2]Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells. Li, Yanmei,Cao, Guangli,Wang, Yang,Xue, Renyu,Gong, Chengliang,Cao, Guangli,Xue, Renyu,Gong, Chengliang,Zhou, Wenlin,Gong, Chengliang.

作者其他论文 更多>>

微信小程序