Overexpression of an Oil Radish Superoxide Dismutase Gene in Broccoli Confers Resistance to Downy Mildew

文献类型： 外文期刊

第一作者： Jiang, Ming

作者： Jiang, Ming;He, Caiming;Miao, Li-xiang

作者机构：

关键词： Superoxide dismutase;Overexpression;Brassica oleracea var. italica;Raphanus sativus var. raphanistroides;Downy mildew

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Superoxide dismutases (SOD) play important roles in plant disease resistance. In this study, a manganese superoxide dismutase gene, designated RsrSOD, was isolated from oil radish (Raphanus sativus var. raphanistroides). RsrSOD was 696 bp in length predicted to encode 231 amino acids. The deduced amino acid sequence contained four putative Mn-binding sites. Under the control of the CaMV35S promoter, RsrSOD was introduced into broccoli (Brassica oleracea var. italica) via Agrobacterium tumefaciens-mediated transformation. Six transgenic lines were obtained out of 35 independent shoots. Both gene expression and enzyme activity of SOD increased significantly in transgenic lines when challenged with Hyaloperonospora parasitica. Three lines, L19, L23, and L25, exhibited the highest resistance against downy mildew with disease symptoms restricted completely. These highly resistant lines would serve as good broccoli breeding materials.

分类号： Q94

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