Cloning and Characterization of Phospholipases A2 and Hyaluronidase Genes from the Venom of the Honeybee Apis mellifera carnica (Hymenoptera: Apidae)

文献类型： 外文期刊

第一作者： Hou, Chunsheng

作者： Hou, Chunsheng;Guo, Liqiong;Wang, Jianrong;You, Linfeng;Lin, Junfang;Wu, Wuhua;Wu, Chengshun;Wang, Teng;Hou, Chunsheng;Guo, Liqiong;Wang, Jianrong;You, Linfeng;Lin, Junfang;Wu, Wuhua;Wu, Chengshun;Wang, Teng;Hou, Chunsheng

作者机构：

关键词： Phospholipase A_2;Hyaluronidase;Honeybee venom;cDNA clone;sequence analysis;Apis mellifera carnica

期刊名称：SOCIOBIOLOGY （ 影响因子：0.983； 五年影响因子：1.01 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Bee venom contains the allergic enzymes phospholipases A2 (PLA2) and hyaluronidase. These enzymes have been extensively studied as therapeutic modalities because of their proven effects in pharmaceutical and clinical applications. The cDNA cloning ofPL A2 and hyaluronidase was amplified by RT-P CR from the total RNA of the venom gland of a honeybee {Apis mellifera carnica). The lengths of the PL A2 and hyaluronidase of Apis mellifera ligustica were 504 and 1 l46bp, respectively. The genes of PLA2 and hyaluronidase shared 90.94% and 96.65% homologies with^. mellifera ligustica and Apis cerana cerana, respectively. Some similar PLA2 and hyaluronidase were also found in the venom of other bee species, We analyzed their sequences and compared them withthose of other sources. A notable finding was that the two genes differed from those of A. mellifera ligustica and A. cerana cerana. The positions of the disulfide bonds of PLA2 and hyaluronidase were also completely different from those previously reported. We used the available sequences to construct a phylogenetic tree and discovered that these two genes of A. mellifera carnica belonged to the western honeybee, and was more closely related to that of A. mellifera ligustica than to any other insect.

分类号： Q

  • 相关文献

[1]Effect of Acetylation of Polyssaccharide from Zizyphus jujuba Fruit on Its alpha-Glycosidase and Hyaluronidase Inhibitory Activities. Jiao Zhonggao,Liu Jie-chao,Zhou Hongping,Wang Sixin. 2009

[2]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[3]Cloning and RNA interference analysis of the salivary protein C002 gene in Schizaphis graminum. Fan Jia,Sun Jing-rui,Chen Ju-lian. 2015

[4]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[5]Cloning of acetyl CoA carboxylase cDNA and the effects of spirodiclofen on the expression of acetyl CoA carboxylase mRNA in Panonychus citri. Yu, Shijiang,Tian, Huixia,Li, Xiaojiao,Yue, Jiansu,Yu, Shijiang,Tian, Huixia,Yang, Juan,Ding, Lili,Chen, Fei,Li, Xiaojiao,Yue, Jiansu,Liu, Haoqiang,Ran, Chun,Chen, Fei.

[6]Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China. Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui,Chen, Jia,Shao, Yun-Hua,Li, Xiang-Dong,Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui. 2009

[7]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[8]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[9]Cloning and Characterization of Goose PPAR gamma Gene Comparison with Other Vertebrates. Wu, H. L.,Kong, F. L.,Qiu, M. H.,Zhou, B.,Luo, J.,Zhu, Q.,Wang, H. Y.,Liu, Y.,He, D. Q.. 2012

[10]Sequence analysis of the haemagglutinin genes of H9N2 avian influenza viruses isolated in China during 1996 to 2011. Dai, Yin,Zhao, Ruihong,Hu, Xiaomiao,Zhang, Danjun,Wang, Junjun,Xu, Fazhi. 2012

[11]Analysis of the gene 3 region sequences of Chinese field strains of Transmissible gastroenteritis virus. Wang, C. B.,Feng, L.,Chen, J. F.,Shi, H. Y.,Qiu, H. J.,Xue, F.,Zhu, Y. M.,Liu, S. W.. 2010

[12]Molecular characterization and expression pattern of the porcine STARS, a striated muscle-specific expressed gene. Peng, Y. B.,Guan, H. P.,Fan, B.,Zhao, S. H.,Xu, X. W.,Li, K.,Zhu, M. J.,Liu, B.,Li, K.,Yerle, M.. 2008

[13]Analysis of S1 gene of avian infectious bronchitis virus isolated in southern China during 2011-2012. Feng, Keyu,Chen, Feng,Xie, Qingmei,Feng, Keyu,Xie, Qingmei,Xue, Yu,Wang, Feng,Shu, Dingming,Xie, Qingmei. 2014

[14]First report of the melon stem rot disease in protected cultivation caused by Pseudomonas fluorescens. Zhang, Chenghua,Wang, Yiwen,Zhu, Pinkuan,Xu, Ling,Lin, Tao,Li, Jufen,Ma, Guobin.

[15]Attenuation of an original US porcine epidemic diarrhea virus strain PC22A via serial cell culture passage. Lin, Chun-Ming,Hou, Yixuan,Gao, Xiang,Liu, Xinsheng,Zheng, Lanlan,Saif, Linda J.,Wang, Qiuhong,Marthaler, Douglas G.,Marthaler, Douglas G.,Liu, Xinsheng,Zheng, Lanlan.

[16]Identification of differentially expressed genes implicated in peel color (red and green) of Dimocarpus confinis. Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan,Jiang, Fan,Chen, Xiu-ping,Hu, Wen-shun,Zheng, Shao-quan. 2016

[17]Characterisation of Hop stunt viroid (HSVd) isolates from jujube trees (Ziziphus jujuba). Liu, GuangYao,Li, Shifang,Zhang, Benli,Wu, Zujian,Jiang, Dongmei,Liu, Chunqin.

[18]Genome sequence determination and analysis of a Chinese virulent strain, LMS, of Gallid herpesvirus type 2. Cheng, Yun,Cong, Feng,Zhang, Yan-ping,Li, Zhi-jie,Xu, Na-na,Hou, Guang-yu,Liu, Chang-jun,Hou, Guang-yu.

[19]Sequence analysis of beta-esterase isoenzymes related to fertility changeover in TsCMS7311 of Chinese cabbage (Brassica rapa L. ssp pekinensis). Zhang, Shaoli,Zhang, Lugang,Zhang, Mingke,Hui, Maixia,Zhang, Shaoli. 2010

[20]Cloning and sequencing of the genome segment s9 of rice black-streaked dwarf virus. Zhang, HM,Chen, JP,Cheng, Y,Lei, JL,Xue, QZ. 2001

作者其他论文 更多>>

微信小程序