文献类型: 外文期刊
第一作者: Yuan, Zhengrong
作者: Yuan, Zhengrong;Li, Junya;Li, Jiao;Zhang, Lupei;Gao, Xue;Gao, Hui Jiang;Xu, Shangzhong;Yuan, Zhengrong
作者机构:
关键词: BRCA1 gene;Cattle;Mastitis;Single nucleotide polymorphisms;Somatic cell count;Somatic cell score
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The main objective of this study was to investigate whether the bovine breast cancer 1 (BRCA1) gene was associated with mastitis resistance in Chinese commercial cattle. A total of 51 SNPs were screened from public data resources and DNA sequencing. Three SNPs (c.5682. G>C,c.26198. C>T and c.46126. G>T) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and created restriction site PCR (CRS-PCR) methods and 21 combinations of these SNPs were observed. The single SNP and their genetic effects on somatic cell score (SCS) were evaluated and a significant association with SCS was found in c.46126. G>T. The mean SCS of individuals with genotype KK was significantly lower than those of genotypes KL and LL. The results of combined genotypes analysis of three SNPs showed that HHLLNN genotype with the highest SCS was easily for the mastitis susceptibility, whereas GGKKMM genotype with the lowest SCS was favorable for the mastitis resistance. The information provided in the present study will be very useful for improving mastitis resistance in dairy cattle by marker-assisted selection (MAS).
分类号: R394
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