A direct assessment of mycotoxin biomarkers in human urine samples by liquid chromatography tandem mass spectrometry

文献类型： 外文期刊

第一作者： Ediage, Emmanuel Njumbe

作者： Ediage, Emmanuel Njumbe;Di Mavungu, Jose Diana;Song, Suquan;Van Peteghem, Carlos;De Saeger, Sarah;Song, Suquan;Wu, Aibo

作者机构：

关键词： Mycotoxin;Biomarkers;Urine;Glucuronide;Experimental design;Stability study

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Detection of mycotoxin biomarkers in urine of humans and animals provides a direct approach for assessing exposure to these mycotoxins as opposed to the indirect approach of food analysis, which in most cases is affected by the heterogeneity of the toxin in the food samples. Seven (7) mycotoxins and their metabolites (total 18 analytes) were selected and an LC-MS/MS method for their determination in human urine was developed and validated. The method consisted of direct analysis of two mycotoxin conjugates, deoxynivalenol-glucuronide and zearalenone-glucuronide without beta glucuronidase digestion of the urine samples. Since high method sensitivity is of utmost importance in such study, critical factors which could improve the analyte recovery and method sensitivity were investigated by a D-optimal experimental design. Urine samples (10mL) were first extracted with 15mL ethyl acetate/formic acid (99/1, v/v) followed by SAX SPE clean-up of the acidified aqueous fraction. Both extracts were combined and analyzed using an LC-MS/MS system operated in the positive ionization mode. A total run time of 28 min was adopted with all the 18 analytes eluting within 15 min. The method was validated by taking into consideration the guidelines specified in Commission Decision 2002/657/EC and 401/2006/EC Forty samples obtained from volunteers within the laboratory research group were analyzed as part of a pilot study. All results were expressed per mg creatinine. A total of 9 samples were found contaminated with one or more of the following analytes: DON, OTA, OTa, 4-OH OTA, ZEN, CFT and β-ZOL One-eighth (5/40) of the samples were contaminated with DON in the range of 3.7-67 ng mg~(-1) creatinine. Samples with detectable levels of DON did not show any co-occurrence of DON-3Glu. One sample was found to be contaminated with 4-OH OTA (

分类号： O65

  • 相关文献

[1]Chemometric-assisted QuEChERS extraction method for the residual analysis of thiacloprid, spirotetramat and spirotetramat's four metabolites in pepper: Application of their dissipation patterns. Liu, Xingang,Dong, Fengshou,Xu, Jun,Xu, Hanqing,Hu, Mingfeng,Zheng, Yongquan. 2016

[2]A statistical approach to determine fluxapyroxad and its three metabolites in soils, sediment and sludge based on a combination of chemometric tools and a modified quick, easy, cheap, effective, rugged and safe method. Liu, Xingang,Zhu, Yulong,Dong, Fengshou,Xu, Jun,Zheng, Yongquan,Li, Minmin.

[3]Sex- and gonad-affecting scent compounds and 3 male pheromones in the rat. Zhang, Jian-Xu,Zhang, Jin-Hua,Sun, Lixing,Feng, Zhi-Yong. 2008

[4]Application of a NMR-based untargeted quantitative metabonomic approach to screen for illicit salbutamol administration in cattle. Tang, Chaohua,Zhang, Kai,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin.

[5]In-tube solid-phase microextraction based on hybrid silica monolith coupled to liquid chromatography-mass spectrometry for automated analysis of ten antidepressants in human urine and plasma. Zheng, Ming-Ming,Wang, Shao-Ting,Hu, Wei-Kang,Feng, Yu-Qi,Zheng, Ming-Ming.

[6]Study on Influences of Enzymolysis and Organic Solvent Extraction on Determination of 2 Kinds of beta(2)-Agonist Residues in Sheep Plasma and Urine. Li Yang,Su Xiao-Ou,Zhang Wei,Fan Xia,Wang Pei-Long,Wang Rui-Guo,Wang Xiao. 2014

[7]Reversed-phase liquid chromatographic determination of cryptotanshinone and its active metabolite in pig plasma and urine. Xue, M,Cui, Y,Wang, HQ,Hu, ZHY,Zhang, B. 1999

[8]Simultaneous detection of dual biomarkers from humans exposed to organophosphorus pesticides by combination of immunochromatographic test strip and ellman assay. Yang, Mingming,Zhao, Yuting,Du, Dan,Lin, Yuehe,Yang, Mingming,Liu, Fengquan,Paulsen, Michael,Simpson, Christopher D.,Wang, Limin. 2018

[9]Molecular cloning and characterization of cat, gpx1 and Cu/Zn-sod genes in pengze crucian carp (Carassius auratus var. Pengze) and antioxidant enzyme modulation induced by hexavalent chromium in juveniles. Li, Meng,Zheng, Yao,Zou, Linhu,Sun, Jiejie,Zhang, Yingying,Qin, Fang,Liu, Shaozhen,Wang, Zaizhao,Liang, Hongwei. 2013

[10]Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin. Li, Z. -H.,Zhong, L. -Q.,Mu, W. -N.,Wu, Y. -H.,Li, Z. -H.,Mu, W. -N.,Wu, Y. -H..

[11]Inhibition and recovery of biomarkers of earthworm Eisenia fetida after exposure to thiacloprid. Zhang, Lan,Zhang, Yanning,Zhang, Pei,Jiang, Hongyun.

[12]Effects of heat stress on serum insulin, adipokines, AMP-activated protein kinase, and heat shock signal molecules in dairy cows. Min, Li,Shi, Bao-lu,Zheng, Nan,Wang, Jia-qi,Min, Li,Yang, Hong-jian,Cheng, Jian-bo. 2015

[13]Isobaric Tags for Relative and Absolute Quantification (iTRAQ)-Based Untargeted Quantitative Proteomic Approach To Identify Change of the Plasma Proteins by Salbutamol Abuse in Beef Cattle. Zhang, Kai,Tang, Chaohua,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin,Zhang, Kai,Tang, Chaohua,Liang, Xiaowei,Zhao, Qingyu,Zhang, Junmin. 2018

[14]Deep sequencing-based identification of pathogen-specific microRNAs in the plasma of rabbits infected with Schistosoma japonicum. Cheng, Guofeng,Luo, Rong,Hu, Chao,Cao, Jie,Jin, Youxin. 2013

[15]Role of microRNAs in schistosomes and schistosomiasis. Zhu, Lihui,Liu, Jinming,Cheng, Guofeng. 2014

[16]New Analytical Tool for the Detection of Ractopamine Abuse in Goat Skeletal Muscle by Potential Gene Expression Biomarkers. Zhao, Luyao,Yang, Shuming,Zhang, Yanhua,Zhang, Ying,Hou, Can,Cheng, Yongyou,You, Xinyong,Tarique, Tunio Muhammad,Gu, Xu,Zhao, Zhen.

[17]Circulating miRNAs: Roles in cancer diagnosis, prognosis and therapy. Cheng, Guofeng.

[18]Obesity Is Associated with Increased Red Blood Cell Folate Despite Lower Dietary Intakes and Serum Concentrations. Bird, Julia K.,Choi, Sang-Woon,Liu, Zhenhua,Ronnenberg, Alayne G.,Choi, Sang-Woon,Du, Fangling,Mason, Joel B.,Mason, Joel B.,Liu, Zhenhua.

[19]Metabolomics analysis reveals that bile acids and phospholipids contribute to variable responses to low-temperature-induced ascites syndrome. Shen, Yiru,Shi, Shourong,Tong, Haibing,Zou, Jianmin,Shi, Shourong,Guo, Yuming.

[20]The Reference Dose for Subchronic Exposure of Pigs to Cadmium Leading to Early Renal Damage by Benchmark Dose Method. Wu, Xiaosheng,Wei, Shuai,Wei, Yimin,Guo, Boli,Zhao, Duoyong,Liu, Xiaoling,Cai, Xianfeng,Wu, Xiaosheng,Yang, Mingqi. 2012

作者其他论文 更多>>

微信小程序