DNA sequence polymorphism of the Rhg4 candidate gene conferring resistance to soybean cyst nematode in Chinese domesticated and wild soybeans

文献类型： 外文期刊

第一作者： Yuan, Cui-Ping

作者： Yuan, Cui-Ping;Li, Ying-Hui;Liu, Zhang-Xiong;Guan, Rong-Xia;Chang, Ru-Zhen;Qiu, Li-Juan

作者机构：

关键词： Glycine max;Glycine soja;Sequence diversity;Heterodera glycines

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Rhg4 is one of the major resistant genes conferring resistance to soybean cyst nematode races 1, 3 and 4. In order to better understand its sequence diversity among different Chinese soybean populations and the impact of human activities on it, we designed 5 primer sets based on its sequence deposited in Genbank (Genbank accession No. AF506518) to obtain the Rhg4 sequence from 104 Chinese cultivated and wild soybean genotypes, and then analyzed the DNA sequence polymorphism in different Chinese soybean populations. The alignment of Rhg4 sequence included 5,216 nucleotide base pairs. A total of 67 single nucleotide polymorphisms (SNPs) including 59 single base changes and 8 DNA insertion-deletions (InDels) were identified with a SNP frequency of 1/78. Except for a 14-base InDel, there were 29 SNPs in coding regions, and among them, 13 were non-synonymous (9 in functional domains with 1 in a leucine-rich repeats region, 2 in a transmembrane region and 6 in a Ser/Thr kinase domain). The probability of substitution at each site was not the same, there were two hot spots, one was in the 5'-untranslated region between positions 124 and 804, and the other was in the region between positions 2520 and 3733. Sequence diversity analysis among 104 soybean genotypes showed pi = 0.00102 and theta = 0.00218 for Rhg4. A domestication bottleneck was found because of lower sequence diversity and 58% unique SNPs loss in landraces compared with Glycine soja. Intensive selection increased the sequence diversity of cultivars, which had higher diversity and more unique SNPs than landraces.

分类号： Q94

  • 相关文献

[1]Cloning and sequence diversity analysis of GmHs1(pro-1) in Chinese domesticated and wild soybeans. Yuan, Cuiping,Zhou, Guoan,Li, Yinghui,Wang, Kejing,Li, Xianghua,Chang, Ruzhen,Qiu, Lijuan,Wang, Zhi. 2008

[2]Analysis of average standardized SSR allele size supports domestication of soybean along the Yellow River. Li, Ying-hui,Zhang, Chen,Li, Wei,Chang, Ru-zhen,Qiu, Li-juan,Smulders, Marinus J. M.,Ma, Yan-song,Xu, Qu.

[3]Identification of quantitative trait loci associated with soybean seed protein content using two populations derived from crosses between Glycine max and Glycine soja. Yan, Long,Xing, Li-Li,Chang, Ru-Zhen,Qiu, Li-Juan,Yan, Long,Yang, Chun-Yan,Zhang, Meng-Chen.

[4]MIC16 gene represents a potential novel genetic marker for population genetic studies of Toxoplasma gondii. Liu, Wen-Ge,Xu, Xiao-Pei,Chen, Jia,Zhu, Xing-Quan,Liu, Wen-Ge,Xu, Xiao-Pei,Xu, Qian-Ming,Luo, Si-Long,Zhu, Xing-Quan. 2016

[5]Sequence variation in ROP8 gene among Toxoplasma gondii isolates from different hosts and geographical localities. Li, Z. Y.,Wang, C. R.,Zhu, X. Q.,Li, Z. Y.,Chen, J.,Lu, J.,Zhu, X. Q.. 2015

[6]Sequence Diversity in MIC6 Gene among Toxoplasma gondii Isolates from Different Hosts and Geographical Locations. Li, Zhong-Yuan,Song, Hui-Qun,Chen, Jia,Zhu, Xing-Quan,Li, Zhong-Yuan,Zhu, Xing-Quan. 2015

[7]Genetic analysis and gene mapping of a narrow leaf mutant in rice (Oryza sativa L.). Wang DeKai,Liu HeQin,Li KeLei,Li SuJuan,Tao YueZhi.

[8]Population dynamics and biocontrol efficacy of the nematophagous fungus Hirsutella minnesotensis in pot assay. Liu, Jianbin,Zhu, Weijing,Hamid, Mohammed Imran,Cheng, Xiaoli,Wang, Niuniu,Sun, Jingzu,Liu, Jianbin.

[9]Parasitism of secondary-stage juvenile of Heterodera glycines and four larva stages of Caenorhabditis elegans by Hirsutella spp. Sun, Jingzu,Xie, Hongyan,Liu, Xingzhong,Xiang, Meichun,Xie, Hongyan,Qiu, Junzhi.

[10]Genetic characteristics of soybean resistance to HG type 0 and HG type 1.2.3.5.7 of the cyst nematode analyzed by genome-wide association mapping. Li, Yinghui,Qiu, Lijuan,Liu, Dongyuan,Zheng, Hongkun,Han, Yingpeng,Zhao, Xue,Cao, Guanglu,Wang, Yan,Teng, Weili,Zhang, Zhiwu,Li, Dongmei,Li, Wenbin. 2015

[11]Analysis of the APX Gene Expressed in Soybean Infected by Heterodera glycines and Coated with Biocontrol Bacteria Sneb545. Xiang, Peng,Li, Hongpeng,Lu, Wencheng,Li, Baohua,Xiang, Peng,Zhu, Feng,Chen, Jingsheng,Li, Hongpeng,Chen, Lijie,Duan, Yuxi,Chen, Jingsheng. 2016

[12]A New Race (X12) of Soybean Cyst Nematode in China. Lian, Yun,Li, Haichao,Wu, Yongkang,Wei, He,Wang, Jinshe,Li, Jinying,Lu, Weiguo,Guo, Jianqiu. 2017

[13]Toxicity of 2,4-diacetylphloroglucinol (DAPG) to Plant-parasitic and Bacterial-feeding Nematodes. Meyer, Susan L. F.,Carta, Lynn K.,Skantar, Andrea M.,Halbrendt, John M.,Liu, Ting,Abdelnabby, Hazem M. E.,Vinyard, Bryan T..

[14]Genetic diversity and differentiation of Chinese wild soybean germplasm (G-soja Sieb. & Zucc.) in geographical scale revealed by SSR markers. Li, X. H.,Wang, K. J.,Jia, J. Z.,Li, X. H.. 2009

[15]Leaf shape polymorphism and its relationship to other characteristics of wild soybean (Glycine soja) in China. Yan, Xuefei,Liu, Shuyuan,Li, Jiandong,Guo, Wei,Sun, Bei,Zhang, Ling,Liu, Xiaodong,Zhao, Hongkun,Gao, Min. 2014

[16]GmPAP3, a novel purple acid phosphatase-like gene in soybean induced by NaCl stress but not phosphorus deficiency. Liao, H,Wong, FL,Phang, TH,Cheung, MY,Li, WYF,Shao, G,Yan, XL,Lam, HM. 2003

[17]A preliminary comparative evaluation of genetic diversity between Chinese and Japanese wild soybean (Glycine soja) germplasm pools using SSR markers. Wang, Ke-Jing,Takahata, Yoshihito. 2007

[18]Identification of a novel variant lacking group A soyasaponin in a Chinese wild soybean (Glycine soja Sieb. & Zucc.): implications for breeding significance. Takahashi, Yuya,Li, Xiang-Hua,Wang, Ke-Jing,Tsukamoto, Chigen. 2016

[19]Categories and components of soyasaponin in the Chinese wild soybean (Glycine soja) genetic resource collection. Takahashi, Yuya,Li, Xiang-Hua,Wang, Ke-Jing,Tsukamoto, Chigen.

[20]Phylogenetic relationships, interspecific hybridization and origin of some rare characters of wild soybean in the subgenus Glycine soja in China. Wang, Ke-Jing,Li, Xiang-Hua.

作者其他论文 更多>>

微信小程序