Extraction and radicals scavenging activity of polysaccharides with microwave extraction from mung bean hulls
文献类型: 外文期刊
第一作者: Zhong, Kui
作者: Zhong, Kui;Lin, Weijing;Wang, Qiang;Zhou, Sumei
作者机构:
关键词: Microwave extraction;Mung bean hulls;Polysaccharides;Radical scavenging activity;Response surface methodology (RSM)
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Extraction variables of microwave extraction mung polysaccharides (MEMPs) from mung bean hulls were optimized by Box-Behnken design. The optimal extraction parameter of MEMP was 700 W, 70 s and 17 mL/g, and highest recovery was 60.03 ± 2.56 mg GE/g DW, which was agreed closely with predicted values. Two purified polysaccharide fractions (MEMP-1 and MEMP-2) were successfully isolated through DEAE cellulose-52 chromatography and Sephadex G-100 size-exclusion chromatography in steps. MEMP-1 was mainly composed of mannose and galactose while that of MEMP-2 was rhamnose and galactose. MEMP and purified fractions both had great radicals scavenging activities of hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and MEMP-2 possessed highest antioxidant activity. The results suggested that MEMP-2 could be the suitable natural antioxidants and may be the functional foods for humans.
分类号: Q71
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