文献类型: 外文期刊
第一作者: Wang, Yong
作者: Wang, Yong;Li, Chuanfeng;Chen, Zongyan;Li, Gang;Wang, Yong;Xu, Binrui;Li, Gang
作者机构:
关键词: Attenuation;Duck hepatitis virus type 1 (DHV-1);Genome comparison
期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Two complete duck hepatitis virus type 1 (DHV-1) genomes, strain SY5 and its chicken embryos passage descendent vaccine strain ZJ-A, were compared and analyzed in order to identify possible sites of attenuation. Of the 205 nucleotide changes, 22 resulted in sense mutations, 174 produced nonsense mutations. Besides, there are 7 consistent nucleotides substitutions in 50UTR and 2 in 30UTR. Three of these 22 sense mutations resided in VP0, 6 exists in VP1, one exists in VP3, 3 exists in 2A2, 3 exists in 2C, one was detected in 3B and 5 was in 3D. These results suggested that VP0, VP1, 3D, and 50/30UTR may contribute to the attenuation of DHV-1 in chicken/ duck/embryos. The results provide a genetic basis for future manipulation of a DHV-1 infectious clone.
分类号: R37
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