Protein serine/threonine phosphotase-1 is essential in governing normal development of vertebrate eye

文献类型： 外文期刊

第一作者： Liu, F. -Y.

作者： Liu, F. -Y.;Tang, X. -C.;Wu, K. -L.;Wu, M. -X.;Liu, X. -L.;Luo, L. -X.;Yu, M. -B.;Liu, Y. -Z.;Li, D. W. -C.;Liu, W. -B.;Yan, Q.;Nie, L.;Zhang, X. -W.;Hu, X. -H.;Hu, W. -F.;Liu, S. -J.;Li, D. W. -C.;Chen, H. -G.;Liu, J.;Ji, W. -K.;Hu, X. -H.;Hu, W. -F.;Woodward, Z.;Li, D. W. -C.;Chen, H. -G.;Liu, J.;Li, D. W. -C.

作者机构：

关键词： Cataract;Differentiation;Eye development;Lens;PP-1;PP-1α/β/γ;Protein phosphatases;Retina;Retina degeneration

期刊名称：CURRENT MOLECULAR MEDICINE （ 影响因子：2.222； 五年影响因子：3.084 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Protein serine/threonine phosphatase-1 (PP-1) is one of the key enzymes responsible for dephosphorylation in vertebrates. Protein dephosphorylation via PP-1 is implicated in many different biological processes including gene expression, cell cycle control, transformation, neuronal transmission, apoptosis, autophage and senescence. However, whether PP-1 directly controls animal development remains to be investigated. Here, we present direct evidence to show that PP-1 plays an essential role in regulating eye development of vertebrates. Using goldfish as a model system, we have shown the following novel results. First, inhibition of PP-1 activity leads to death of a majority of the treated embryos, and the survived embryos displayed severe phenotype in the eye. Second, knockdown of each catalytic subunit of PP-1 with morpholino oligomers leads to partial (PP-lγ knockdown) or complete (PP-lγ or PP-lγ knockdown) death of the injected embryos. The survived embryos from PP-1γ knockdown displayed clear retardation in lens differentiation. Finally, overexpression of each subunit of PP-1 also causes death of majority of the injected embryos and leads to abnormal development of goldfish eye. Mechanistically, Pax-6 is one of the major downstream targets mediating the effects of PP-1 function since the eye phenotype in Pax-6 knockdown fish is similar to that derived from overexpression of PP-1. Together, our results for the first time provide direct evidence that protein phosphatase-1 plays a key role in governing normal eye formation during goldfish development.

分类号： Q7

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