Shape evolution with temperature of a thermotolerant protein (PeaT1) in solution detected by small angle X-ray scattering

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Zhang, Kunhao;Cai, Quan;Mo, Guang;Cheng, Weidong;Wang, Dehong;Gong, Yu;Chen, Zhongjun;Wu, Zhonghua;Xing, Xueqing;Cheng, Weidong;Wang, Dehong;Gong, Yu;Wu, Zhonghua;Liu, Quan;Li, Tang;Qiu, Dewen;Liu, Quan

作者机构：

关键词： Domain;PeaT1;SAXS;SRCD;Structure prediction;Structure restoration

期刊名称：PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS （ 影响因子：3.756； 五年影响因子：2.752 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The protein elicitor from Alternaria tenuissima (PeaT1) presented excellent thermotolerance and potential application in agriculture as a pesticide. Previous synchrotron radiation circular dichroism study demonstrated that the secondary structures in PeaT1 protein are reversible with temperature change. To further clarify the mechanism of its thermotolerance, synchrotron radiation small angle x-ray scattering (SAXS) technique was used to study the shape change of PeaT1 protein with temperature in this article. Ab initio structure restorations based on the SAXS data revealed that PeaT1 protein has a prolate shape with a P2 symmetry axis along the prolate anisometric direction. With temperature increase, a gooseneck vase-like (25°C), to jug-like (55°C), then to oval (85°C) shape change can be found, and these shape changes are also approximately reversible with temperature decrease. PeaT1 protein contains two homogenous molecules, and each of them consists of F, NAC, T, and UBA domains. The structures of the four domains were predicted. Simulated annealing algorithm was used to superimpose the domain structures onto the SAXS shapes. It was found that all the structural domains have position rotation and translation with temperature change, but the NAC domains are relatively stable, playing a role of frame. This shape change information provides clues for further exploring its biological function and application.

分类号： Q5

  • 相关文献

[1]Stable isotope labelled mass spectrometry for quantification of the relative abundances for expressed proteins induced by PeaT1. Yang XiuFen,Zeng HongMei,Mao JianJun,Liu Hua,Zhang YunHua,Qiu DeWen. 2010

[2]Overexpression of the PeaT1 Elicitor Gene from Alternaria tenuissima Improves Drought Tolerance in Rice Plants via Interaction with a Myo-Inositol Oxygenase. Dong, Yijie,Zhang, Yi,Yang, Xiufeng,Qiu, Dewen,Shi, Fachao. 2017

[3]The effect of lamellar structure ordering on the retrogradation properties of canna starch subjected to thermal and enzymatic degradation. Lan, Xiaohong,Yang, Ying,Wu, Jinhong,Wang, Zhengwu,Liu, Xingxun.

[4]Lamellar structure change of waxy corn starch during gelatinization by time-resolved synchrotron SAXS. Kuang, Qirong,Xu, Jinchuan,Zhou, Sumei,Liu, Xingxun,Liang, Yongri,Xie, Fengwei,Tian, Feng. 2017

[5]Dimer conformation of soluble PECAM-1, an endothelial marker. Jiang, Longguang,Huang, Mingdong,Jiang, Longguang,Yuan, Cai,Xu, Mingming,Huang, Mingdong,Lin, Lin,Li, Rui,Huang, Joy H..

[6]Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides. Liu, YS,Zhang, J,Liu, Q,Zhang, CG,Ma, QS.

[7]Genome-wide analysis of SINA family in plants and their phylogenetic relationships. Wang, Meng,Jin, Ying,Fu, Junjie,Zhu, Yun,Hu, Jian,Wang, Guoying,Zheng, Jun,Wang, Guoying.

[8]Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Eschetichia coli and transgenic tobacco. Dun, Bao-Qing,Wang, Xu-Jing,Lu, Wei,Zhao, Zhong-Lin,Hou, Song-Na,Lin, Min,Dun, Bao-Qing,Zhang, Bao-Ming,Li, Gui-Ying,Zhao, Zhong-Lin,Evans, Thomas C., Jr.,Xu, Ming-Qun.

[9]Fusion expression of bovine lactoferricin in Escherichia coli. Feng, Xing-jun,Wang, Jian-hua,Shan, An-shan,Teng, Da,Yang, Ya-lin,Yao, Yi,Yang, Guan-pin,Shao, Yan-chun,Liu, Shuo,Zhang, Fan.

[10]The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana. Pei, Xinwu,Wang, Zhixing,Jia, Shirong,Zhang, Yonggiang,Li, Weimin,Guo, Shiwei.

作者其他论文 更多>>

微信小程序