Mutation of a vitelline membrane protein, BmEP80, is responsible for the silkworm "Ming" lethal egg mutant

文献类型： 外文期刊

第一作者： Chen, Anli

作者： Chen, Anli;Gao, Peng;Zhao, Qiaoling;Tang, Shunming;Shen, Xingjia;Zhang, Guozheng;Qiu, Zhiyong;Xia, Dingguo;Chen, Anli;Gao, Peng;Zhao, Qiaoling;Tang, Shunming;Shen, Xingjia;Zhang, Guozheng;Qiu, Zhiyong;Xia, Dingguo;Huang, Yongping;Xu, Yunmin;He, Ningjia

作者机构：

关键词： BmEP80;BmEP80 protein;Bombyx mori;Lethal egg mutant;Positional cloning

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The egg stage is an important stage in the silkworm (Bombyx mori) life cycle. Normal silkworm eggs are usually short, elliptical, and laterally flattened, with a sometimes hollowed surface on the lateral side. However, the eggs laid by homozygous recessive "Ming" lethal egg mutants (l-em) lose water and become concaved around 1h, ultimately exhibiting a triangular shape on the egg surfaces. We performed positional cloning, and narrowed down the region containing the gene responsible for the l-em mutant to 360kb on chromosome 10 using 2287 F2 individuals. Using expression analysis and RNA interference, the best l-em candidate gene was shown to be BmEP80. The results of the inverse polymerase chain reaction showed that an ~1.9kb region from the 3' untranslated region of BmVMP23 to the forepart of BmEP80 was replaced by a >100kb DNA fragment in the l-em mutant. Several eggs laid by the normal moths injected with BmEP80 small interfering RNAs were evidently depressed and exhibited a triangular shape on the surface. The phenotype exhibited was consistent with the eggs laid by the l-em mutant. Moreover, two-dimensional gel electrophoresis showed that the BmEP80 protein was expressed in the ovary from the 9th day of the pupa stage to eclosion in the wild-type silkworm, but was absent in the l-em mutant. These results indicate that BmEP80 is responsible for the l-em mutation. ? 2012 Elsevier B.V.

分类号： R394

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