CDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi

文献类型： 外文期刊

第一作者： Xia, Dingguo

作者： Xia, Dingguo;Xiang, Zhonghuai;Lu, Cheng;Xia, Dingguo;Wei, Yadong;Zhang, Guozheng;Zhao, Qiaoling;Zhang, Yeshun

作者机构：

关键词： Batocera horsfieldi;Beetle;CDNA cloning;Cellulase;Enzymatic activity;Insect

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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年卷期：

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收录情况： SCI

摘要： In this study, we report a novel cellulase [??-1,4-endoglucanase (EGase), EC 3.2.1.4] cDNA (Bh-EGase II) belonging to the glycoside hydrolase family (GHF) 45 from the beetle Batocera horsfieldi. The Bh-EGase II gene spans 720. bp and consists of a single exon coding for 239 amino acid residues. Bh-EGase II showed 93.72% protein sequence identity to Ag-EGase II from the beetle Apriona germari. The GHF 45 catalytic site is conserved in Bh-EGase II. Bh-EGase II has three putative N-glycosylation sites at 56-58 (N-K-S), 99-101 (N-S-T), and 237-239 (N-Y-S), respectively. The cDNA encoding Bh-EGase II was expressed in baculovirus-infected insect BmN cells and Bombyx mori larvae. Recombinant Bh-EGase II from BmN cells and larval hemolymph had an enzymatic activity of approximately 928. U/mg. The enzymatic catalysis of recombinant Bh-EGase II showed the highest activity at 50. ??C and pH. 6.0. ? 2012.

分类号： R394

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