CDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi
文献类型: 外文期刊
第一作者: Xia, Dingguo
作者: Xia, Dingguo;Xiang, Zhonghuai;Lu, Cheng;Xia, Dingguo;Wei, Yadong;Zhang, Guozheng;Zhao, Qiaoling;Zhang, Yeshun
作者机构:
关键词: Batocera horsfieldi;Beetle;CDNA cloning;Cellulase;Enzymatic activity;Insect
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this study, we report a novel cellulase [??-1,4-endoglucanase (EGase), EC 3.2.1.4] cDNA (Bh-EGase II) belonging to the glycoside hydrolase family (GHF) 45 from the beetle Batocera horsfieldi. The Bh-EGase II gene spans 720. bp and consists of a single exon coding for 239 amino acid residues. Bh-EGase II showed 93.72% protein sequence identity to Ag-EGase II from the beetle Apriona germari. The GHF 45 catalytic site is conserved in Bh-EGase II. Bh-EGase II has three putative N-glycosylation sites at 56-58 (N-K-S), 99-101 (N-S-T), and 237-239 (N-Y-S), respectively. The cDNA encoding Bh-EGase II was expressed in baculovirus-infected insect BmN cells and Bombyx mori larvae. Recombinant Bh-EGase II from BmN cells and larval hemolymph had an enzymatic activity of approximately 928. U/mg. The enzymatic catalysis of recombinant Bh-EGase II showed the highest activity at 50. ??C and pH. 6.0. ? 2012.
分类号: R394
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