Poly(C)-Binding Protein 1, a Novel N-pro-Interacting Protein Involved in Classical Swine Fever Virus Growth

文献类型： 外文期刊

第一作者： Li, Dan

作者： Li, Dan;Li, Su;Sun, Yuan;Dong, Hong;Li, Yongfeng;Zhao, Bibo;Guo, Dongwei;Weng, Changjiang;Qiu, Hua-Ji

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期刊名称：JOURNAL OF VIROLOGY （ 影响因子：5.103； 五年影响因子：5.078 ）

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收录情况： SCI

摘要： N-pro is a multifunctional autoprotease unique to pestiviruses. The interacting partners of the N-pro protein of classical swine fever virus (CSFV), a swine pestivirus, have been insufficiently defined. Using a yeast two-hybrid screen, we identified poly(C)-binding protein 1 (PCBP1) as a novel interacting partner of the CSFV N-pro protein and confirmed this by coimmunoprecipitation, glutathione S-transferase (GST) pulldown, and confocal assays. Knockdown of PCBP1 by small interfering RNA suppressed CSFV growth, while overexpression of PCBP1 promoted CSFV growth. Furthermore, we showed that type I interferon was downregulated by PCBP1, as well as N-pro. Our results suggest that cellular PCBP1 positively modulates CSFV growth.

分类号： Q934.2

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