Identification and characterization of PtDGAT2B, an acyltransferase of the DGAT2 acyl-Coenzyme A: Diacylglycerol acyltransferase family in the diatom Phaeodactylum tricornutum

文献类型： 外文期刊

第一作者： Gong, Yangmin

作者： Gong, Yangmin;Zhang, Junping;Guo, Xiaojing;Wan, Xia;Liang, Zhuo;Hu, Chuan Jiong;Jiang, Mulan;Zhang, Junping

作者机构：

关键词： DGAT2;Fatty acid;Phaeodactylum tricornutum;Triacylglycerol;Yeast

期刊名称：FEBS LETTERS （ 影响因子：4.124； 五年影响因子：3.814 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Diacylglycerol acyltransferase (DGAT) plays a pivotal role in triacylglycerol (TAG) formation in some oleaginous organisms. We describe here the identification of a type 2 DGAT (PtDGAT2B) in the diatom Phaeodactylum tricornutum that contains four putative type 2 acyl-CoA:DGATs, sharing little sequence similarity with each other. TAG synthesis and lipid body formation could be completely restored in a Saccharomyces cerevisiae TAG-deficient quadruple mutant by expressing PtDGAT2B. Up-regulation of PtDGAT2B precedes the accumulation of TAG. Functional analysis of enzyme activity in vivo demonstrated that expression of PtDGAT2B can increase the proportion of unsaturated C 16 and C18 fatty acids in yeast TAG. ? 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

分类号： Q5

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