Molecular cloning and characterization of SoNCED, a novel gene encoding 9-cis-epoxycarotenoid dioxygenase from sugarcane (Saccharum officinarum L.)

文献类型： 外文期刊

第一作者： Nong, Qian

作者： Nong, Qian;Yang, Li-Tao;Li, Yang-Rui;Li, Chang-Ning;Srivastava, Manoj-Kumar;Yang, Li-Tao;Li, Yang-Rui;Li, Chang-Ning;Srivastava, Manoj-Kumar;Yang, Li-Tao;Li, Yang-Rui

作者机构：

关键词： Cloning;Gene expression;NCED;ROS;Sugarcane

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) plays important roles in adaptive responses to various environmental stresses. The rate-limiting step in ABA biosynthesis is the oxidative cleavage of cis-epoxycarotenoids, which is catalyzed by 9-cis-epoxycarotenoid dioxygenase (NCED). In this experiment, a full-length cDNA encoding NCED gene was cloned by RT-PCR and RACE from sugarcane (Saccharum officinarum L.). The full-length of SoNCED is 2,521 bp with 1,827 bp open reading frame, encoding a peptide of 608 amino acids. The calculated molecular weight of protein was 65.9 kDa with isoelectric point of 6.04. Conserved domains prediction indicated a chloroplast-targeting peptide located at N-terminus of SoNCED. Phylogenetic tree, constructed by Neighbor-Ioining method indicatedthat SoNCED shared high identity with the NCEDs reported from other plant species. Sequence alignment revealed that the basic secondary structure including alpha, beta-strands, beta-propeller and His residues coordinating catalytic sites of SoNCED were highly conserved as in the NCEDs from other plants. Tissue specific expression analysis using quantitative real-time PCR showed a significant increase in SoNCED mRNA level and its correlation with O_2- production rate and ABA accumulation in leaves and roots of sugarcane variety GT21 when exposed to water stress. Further, the stimulation of SoNCED mRNA level, O_2 production rate and ABA content after exogenous application of ABA (100 muMol l~(-1)) proved its involvement in pathways providing tolerance todrought stress.

分类号： Q

  • 相关文献

[1]Molecular Cloning and Functional Analysis of an Ethylene Receptor Gene from Sugarcane (Saccharum spp.) by Hormone and Environmental Stresses. Yang, Li-Tao,Li, Yang-Rui,Wang, Ai-Qin,Yang, Li-Tao,Li, Yang-Rui,Wang, Ai-Qin,Ye, Xing-Zhi,Huang, Jing-Li,Niu, Jun-Qi,Liu, Ming,Yang, Li-Tao,Li, Yang-Rui,Pan, Yong-Bao. 2015

[2]Transcriptome of High-Sucrose Sugarcane Variety GT35. Gao, Yi-Jing,Gui, Yi-Yun,Chen, Zhong-Liang,Qin, Cui-Xian,Wang, Miao,Li, Yang-Rui,Liao, Qing,Li, Yang-Rui,Yang, Li-Tao. 2016

[3]Role of the SPS Gene Families in the Regulation of Sucrose Accumulation in Sugarcane. Qin, Cui-Xian,Gui, Yi-Yun,Chen, Zhong-Liang,Wang, Miao,Liao, Qing,Li, Yang-Rui,Zhao, Li-Han,Lakshmanan, Prakash. 2017

[4]Isolation, Characterization and Promoter Analysis of Cell Wall Invertase Gene SoCIN1 from Sugarcane (Saccharum spp.). Wang, Ai-Qin,Huang, Jing-Li,Yang, Li-Tao,Yang, Li-Tao,Li, Yang-Rui,Yang, Li-Tao,Li, Yang-Rui. 2015

[5]Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response. Feng, Juan. 2012

[6]Cloning, expression analysis, and antibacterial propertiesof three serum amyloid A in common carp (Cyprinus carpio). Yu, Juhua,Tang, Yongkai,Li, Jianlin,Li, Hongxia,Yu, Fan,Yu, Juhua,Yu, Wenjuan,He, Feng,Mao, Shuntao,Fu, Chunjie.

[7]Functional markers in wheat: current status and future prospects. Liu, Yanan,He, Zhonghu,Xia, Xianchun,He, Zhonghu,Appels, Rudi.

[8]Cloning and characterization of fatty acid transport proteins in Japanese seabass Lateolabrax japonicus, and their gene expressions in response to dietary arachidonic acid. Xu, Houguo,Zhang, Yuanqin,Wang, Chengqiang,Wei, Yuliang,Zheng, Keke,Liang, Mengqing,Xu, Houguo,Zheng, Keke,Liang, Mengqing,Zhang, Yuanqin.

[9]Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis. Ye, Huan,Du, Hao,Chen, Xi-Hua,Liu, Tao,Li, Chuang-Ju,Ye, Huan,Liu, Tao,Du, Hao,Li, Chuang-Ju,Cao, Hong.

[10]Cloning and expression of genes related to the sucrose-metabolizing enzymes and carbohydrate changes in peach. Zhang, Chunhua,Shen, Zhijun,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Korir, Nicholas Kibet.

[11]Cloning and expression of UDP-glucose: flavonoid 3-O-glucosyltransferase gene in peach flowers. Wen, X. C.,Han, J.,Leng, X. P.,Jiang, W. B.,Fang, J. G.,Ma, R. J.. 2014

[12]Molecular cloning and expression analysis of TRAF3 in chicken. Yang, H. L.,Feng, Z. Q.,Zeng, S. Q.,Zhu, Q.,Liu, Y. P.,Li, S. M.. 2015

[13]The co-existence of two growth hormone receptors and their differential expression profiles between female and male tongue sole (Cynoglossus semilaevis). Ma, Qian,Liu, Shu-Fang,Zhuang, Zhi-Meng,Sun, Zhong-Zhi,Liu, Chang-Lin,Tang, Qi-Sheng,Ma, Qian.

[14]Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis. Li, Chuang-Ju,Chen, Xi-Hua,Liu, Zhi-Gang,Wei, Qi-Wei,Tang, Yong-Kai,Li, Chuang-Ju,Gan, Fang,Chen, Xi-Hua,Liu, Zhi-Gang,Li, Luo-Xin,Wei, Qi-Wei.

[15]Characterization and Transcriptional Analysis of a New CC Chemokine Associated with Innate Immune Response in Cobia (Rachycentron canadum). Su, Y.,Feng, J.,Xu, L.,Jiang, J.,Sun, X.,Guo, Z..

[16]Role of abscisic acid and ethylene in sweet cherry fruit maturation: molecular aspects. Ren, J.,Chen, P.,Dai, S. J.,Li, P.,Li, Q.,Ji, K.,Wang, Y. P.,Leng, P.,Ren, J.. 2011

[17]The Role of ABA in the Maturation and Postharvest Life of a Nonclimacteric Sweet Cherry Fruit. Luo, Hao,Dai, ShengJie,Ding, Ying,Sun, Yufei,Ji, Kai,Wang, Yanping,Li, Qian,Chen, Pei,Duan, Chaorui,Wang, Ya,Leng, Ping,Ren, Jie,Zhang, CaiXia,Li, Zhuang. 2014

[18]Histological changes and differences in activities of some antioxidant enzymes and hydrogen peroxide content during somatic embryogenesis of Musa AAA cv. Yueyoukang 1. Jiang, Shuang,Chen, Houbin,Xu, Chunxiang,Xie, Lijun,Li, Huaping,Lin, Guimei,Takac, Tomas,Samaj, Jozef,Samaj, Jozef. 2012

[19]Dose dependent rhizospheric Ni toxicity evaluation: Membrane stability and antioxidant potential of Vigna species. Mahmood, Seema,Ishtiaq, Shabnam,Yasin, Ghulam,Irshad, Ahsan. 2016

[20]TIP60 contributes to porcine embryonic development by regulating DNA damage response. Guo, Jing,Zhou, Wenjun,Niu, Ying-Jie,Shin, Kyung-Tae,Kim, Nam-Hyung,Cui, Xiang-Shun,Guo, Jing,Heo, Young Tae. 2018

作者其他论文 更多>>

微信小程序