Expression of the alfalfa FRIGIDA-Like Gene, MsFRI-L delays flowering time in transgenic Arabidopsis thaliana
文献类型: 外文期刊
第一作者: Chao, Yuehui
作者: Chao, Yuehui;Yang, Qingchuan;Kang, Junmei;Zhang, Tiejun;Sun, Yan
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关键词: MsFRI-L;FRIGIDA-Like Gene;Flowering time;Transgenic Arabidopsis thaliana
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
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收录情况: SCI
摘要: In Arabidopsis, allelic variation at the FRIGIDA (FRI) locus is a major determinant of natural variation in flowering time. We have identified a FRIGIDA like protein gene, MsFRI-L, with unknown function from Medicago sativa L. Expression of MsFRI-L was increased in wild alfalfa under continuous cold conditions and the gene was expressed in all tissues including roots, stems, leaves and flowers, and weakest in flowers under light/dark conditions. Expression of MsFRI-L in transgenic Arabidopsis plantsresulted in late flowering phenotypes under long day conditions or treated with vernalization. Subcellular analysis showed that MsFRI-L was localized in the nucleus. Transcript profiling of floral regulatory genes in MsFRI-L transgenic Arabidopsis showedenhanced expression of the flowering repressor FLOWERING LOCUS C and decreased expression of one key flowering time genes FLOWERING LOCUS T. These results suggest that MsFRI-L delays flowering time by regulating flowering genes expression under long dayconditions.
分类号: Q7
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