Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice

文献类型: 外文期刊

第一作者: Yin, Xiangping

作者: Yin, Xiangping;Li, Zhiyong;Li, Jiangtao;Zhang, Yun;Li, Xuerui;Li, Baoyu;Yang, Bin;Lan, Xi;Jiao, Wenqiang;Liu, Jixing;Yi, Yongzhu;Li, Yinv;Zhang, Zhifang

作者机构:

关键词: Rabies virus;Nucleoprotein;Recombinant silkworm baculovirus;Vaccine

期刊名称:JOURNAL OF BIOTECHNOLOGY ( 影响因子:3.307; 五年影响因子:3.778 )

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收录情况: SCI

摘要: Rabies is one of the most fatal zoonotic diseases in developing countries, where a safe, cheap and effective vaccine against the disease remains unaffordable. In this paper, we describe a new silkworm-baculovirus expression system to express the nucleoprotein (N) gene of rabies virus and evaluation of the immune response in BALB/c mice. A recombinant baculovirus -rBmNPV(RV-N) carrying the N gene of rabies virus Evelyn Rokitniki Abelseth (ERA) strain was constructed and the N protein expression was evaluated in Bombyx mori (BmN) cells and silkworm pupae by immunofluorescence staining, Western blots and enzyme-linked immunosorbent assay (ELISA). The immune response to vaccines was evaluated based on serum IgG antibody titers and challenge experiments. The study revealed that N protein of rabies virus can be highly expressed in silkworm baculovirus expression system and the vaccine of N antigen presents a promising approach for the prevention of rabies virus

分类号: Q81

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