Phage-Displayed Peptide That Mimics Aflatoxins and Its Application in Immunoassay

文献类型: 外文期刊

第一作者: Wang, Yanru

作者: Wang, Yanru;Zhang, Qi;Wang, Yanru;Li, Peiwu;Wang, Yanru;Li, Peiwu;Zhang, Qi;Wang, Yanru;Li, Peiwu;Wang, Hong;Kim, Hee Jou;Gee, Shirley J.;Hammock, Bruce D.;Wang, Hong;Kim, Hee Jou;Gee, Shirley J.;Hammock, Bruce D.

作者机构:

关键词: aflatoxin;phage-disptayed peptide;phage peptide library;mimotope;matrix effect

期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random- 8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by pannmg-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC_(50) value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.

分类号: R15

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