MRT letter: Localization of endogenous hydrogen peroxide by modified processes of sample preparation for transmissionelectron microscope in Escherichia coli

文献类型： 外文期刊

第一作者： Hu, Rongliu

作者： Hu, Rongliu;Zhu, Wenxue;Fan, Jinling;Wang, Na;Wang, Liping;Yang, Lipeng;Li, Xin;He, Chenyang;Pang, Xinyue;Zhao, Chunyan

作者机构：

关键词： Escherichia coli;Fixation;Hydrogen peroxide;Staining;Transmission electron microscope

期刊名称：MICROSCOPY RESEARCH AND TECHNIQUE （ 影响因子：2.769； 五年影响因子：2.11 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The bacterial endogenous hydrogen peroxide (H_2O_2) was detected cytochemically by its reaction with cerium chloride (CeCl_3) to produce electron-dense deposits of cerium perhydroxides. The sequence of fixation and CeCl_3 staining of H_2O_2 in the processing of transmission electron microscope (TEM) sample preparation is crucial to the localization of endogenous H_2O_2 in Escherichia coli. In this study, results confirmed that the process that fixation simultaneously with CeCl_3 staining provided optimum effects for H_2O_2 localization in E. coli. The modified process of TEM provides very efficient protection for H_2O_2 localization and more accurate quantization for the H_2O_2 accumulation in bacterial cells.

分类号： TH742

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