Quantitative proteomics by amino acid labeling in foot-and-mouth disease virus (FMDV)-infected cells

文献类型： 外文期刊

第一作者： Ye, Yu

作者： Ye, Yu;Xin, Chaoan;Liao, Ming;Fan, Huiying;Ye, Yu;Luo, Yongwen;Xin, Chaoan;Liao, Ming;Fan, Huiying;Ye, Yu;Xin, Chaoan;Liao, Ming;Fan, Huiying;Tong, Tiezhu;Yan, Guangrong;Yan, Guangrong;Liu, Xiangtao

作者机构：

关键词： Foot-and-mouth disease virus (FMDV);Pathway analysis;Quantitative proteomics;Stable isotope labeling with amino acids in cell culture (SILAC)

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Foot-and-mouth disease virus (FMDV) is an important disease agent that can be difficult to effectively eradicate from herds. Because it is an obligate intracellular parasite, the virus has multiple effects on the host cell during infection. Here, a high-throughput quantitative proteomic approach was used to develop an unbiased holistic overview of the protein changes in IBRS-2 cells infected with FMDV. Stable isotope labeling with amino acids in cell culture (SILAC) combined with LC-MS/MS was performed to identify and quantify 1260 cellular and 2 viral proteins after 6 h of infection of IBRS-2 cells with FMDV. Of these identified and measured cellular protein pairs, 77 were significantly up-regulated, and 50 were significantly down-regulated based on significance B ≥ 0.05. The differentially altered proteins included a number of proteins involved in endolysosomal proteases system, cell cycle, cellular growth and proliferation, and immune cell trafficking. Selected data were validated by Western blot. Ingenuity Pathway Analysis revealed that proteins that changed in response to infection could be assigned to defined canonical pathways and functional groupings, such as integrin signaling. The obtained data might not only improve the understanding of the dynamics of FMDV and host interaction but may also help elucidate the pathogenic mechanism of FMDV infection.

分类号： Q7

  • 相关文献

[1]Comparative Proteomic Analysis of Wild-Type and SAP Domain Mutant Foot-and-Mouth Disease Virus-Infected Porcine Cells Identifies the Ubiquitin-Activating Enzyme UBE1 Required for Virus Replication. Zhu, Zixiang,Yang, Fan,Zhang, Keshan,Cao, Weijun,Jin, Ye,Wang, Guoqing,Mao, Ruoqing,Li, Dan,Guo, Jianhong,Liu, Xiangtao,Zheng, Haixue.

[2]Whole genome re-sequencing and transcriptome analysis of the Stylosanthes Anthracnose pathogen Colletotrichum gloeosporioides reveal its characteristics. Huang, H. P.,Huang, H. P.,Huang, J. H.,Zheng, J. L.,Yi, K. X.,Ma, S.. 2016

[3]Transcriptional profiles in bursal B-lymphoid DT40 cells infected with very virulent infectious bursal disease virus. Quan, Rong,Zhu, Shanshan,Wei, Li,Wang, Jing,Yan, Xu,Li, Zixuan,Liu, Jue. 2017

[4]The potential molecular effects of bursal septpeptide II on immune induction and antitumor activity. Zhou, Guang Fang,Zhou, Bin,Liu, Xiao Dong,Feng, Xiu Li,Cao, Rui Bing,Chen, Pu Yan,Liu, Qing Tao,Qiu, Ya Feng,Ma, Zhi Yong.

[5]Differential gene expression in porcine SK6 cells infected with wild-type and SAP domain-mutant foot-and-mouth disease virus. Ni, Zixin,Yang, Fan,Cao, Weijun,Zhang, Xiangle,Jin, Ye,Mao, Ruoqing,Du, Xiaoli,Li, Weiwei,Guo, Jianhong,Liu, Xiangtao,Zhu, Zixiang,Zheng, Haixue,Ni, Zixin. 2016

[6]Molecular relationships between type Asia 1 new strain from China and type O Panasia strains of foot-and-mouth-disease virus. Li, Dong,Shang, You-Jun,Shang, Jun,Liu, Zai-Xin,Liu, Xiang-Tao,Cai, Xue-Peng. 2007

[7]High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts. Li, Y,Sun, M,Liu, JX,Yang, ZQ,Zhang, ZF,Shen, GF. 2006

[8]The rescue and evaluation of FLAG and HIS epitope-tagged Asia 1 type foot-and-mouth disease viruses. Yang, Bo,Yang, Fan,Zhang, Yan,Liu, Huanan,Jin, Ye,Cao, Weijun,Zhu, Zixiang,Zheng, Haixue,Yin, Hong.

[9]Development of a serotype colloidal gold strip using monoclonal antibody for rapid detection type Asia1 foot-and-mouth disease. Lin, Tong,Shao, Jun-jun,Du, Jun-zheng,Cong, Guo-zheng,Gao, Shan-dian,Chang, Huiyun. 2011

[10]The effect of bovine IFN-alpha on the immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus. Guo, HC,Liu, ZX,Sun, SQ,Leng, QW,Li, D,Liu, XT,Xie, QG. 2004

[11]The infectivity and pathogenicity of a foot-and-mouth disease virus persistent infection strain from oesophageal-pharyngeal fluid of a Chinese cattle in 2010. Bao, Hui-Fang,Li, Dong,Sun, Pu,Zhou, Qiang,Bai, Xing-Wen,Fu, Yuan-Fang,Lu, Zeng-Jun,Liu, Zai-Xin,Hu, Jun. 2011

[12]Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum). He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang,Li, Yi-Na,Zhang, Zhi-Fang,Su, Zhong-Liang,Shao, Hong-Bo.

[13]Comparative analysis of primary hepatocellular carcinoma with single and multiple lesions by iTRAQ-based quantitative proteomics. Xing, Xiaohua,Huang, Yao,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Xing, Xiaohua,Wang, Sen,Chi, Minhui,Zeng, Yongyi,Chen, Lihong,Li, Ling,Zeng, Jinhua,Liu, Minjie,Liu, Xiaolong,Liu, Jingfeng,Han, Xiao.

[14]Proteomic characterization and comparison of mammalian milk fat globule proteomes by iTRAQ analysis. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Ma, Lu,Zhao, Shengguo,Li, Songli,Guo, Tongjun,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Zheng, Nan,Zhang, Yangdong,Zhao, Shengguo,Li, Songli,Wang, Jiaqi,Han, Rongwei.

[15]Proteome Differences between Hepatitis B Virus Genotype-B- and Genotype-C-Induced Hepatocellular Carcinoma Revealed by iTRAQ-Based Quantitative Proteomics. Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Wei, Dahai,Zeng, Yongyi,Xing, Xiaohua,Liu, Hongzhi,Lin, Minjie,Liu, Xiaolong,Liu, Jingfeng,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao,Liu, Xiaolong,Liu, Jingfeng.

[16]Reveal the molecular signatures of hepatocellular carcinoma with different sizes by iTRAQ based quantitative proteomics. Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Wang, Yingchao,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Xing, Xiaohua,Xia, Jiangbao,Lin, Minjie,Liao, Naishun,Liu, Xiaolong,Liu, Jingfeng,Liu, Hongzhi,Liang, Dong,Huang, Yao,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao.

[17]Differential proteomic analysis of rice seedlings reveals the advantage of dry-raising nursery practices. Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Zhang, Zhixing,Huang, Fenglian,Chen, Hongfei,Lin, Wenxiong,Shao, CaiHong. 2018

[18]Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann. Ji, Ting,Liu, Zhenguo,Shen, Jie,Shen, Fang,Chen, Guohong,Liang, Qin,Wu, Liming,Corona, Miguel. 2014

[19]Proteomic analysis of sheep primary testicular cells infected with bluetongue virus. Du, Junzheng,Xing, Shanshan,Tian, Zhancheng,Gao, Shandian,Xie, Junren,Chang, Huiyun,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong.

[20]Quantitative proteomics of Sesuvium portulacastrum leaves revealed that ion transportation by V-ATPase and sugar accumulation in chloroplast played crucial roles in halophyte salt tolerance. Yi, Xiaoping,Sun, Yong,Yang, Qian,Guo, Anping,Chang, Lili,Wang, Dan,Tong, Zheng,Jin, Xiang,Wang, Limin,Wang, Xuchu,Yi, Xiaoping,Guo, Anping,Chang, Lili,Wang, Xuchu,Yu, Jianlan,Jin, Wenhai.

作者其他论文 更多>>

微信小程序