Cloning and characterization of a cathepsin L-like cysteine protease from Taenia pisiformis

文献类型: 外文期刊

第一作者: Wang, Qiuxia

作者: Wang, Qiuxia;Zhang, Shaohua;Luo, Xuenong;Hou, Junling;Zhu, Xueliang;Cai, Xuepeng

作者机构:

关键词: Taenia pisiformis metacestode;Cysteine protease;Recombinant protein;Biochemical characteristics

期刊名称:VETERINARY PARASITOLOGY ( 影响因子:2.738; 五年影响因子:2.951 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Rabbit cysticercosis, caused by the larval stage of Taenia pisiformis, is a serious parasitic disease of rabbits. It was reported that some cysteine peptidases have potential roles in-the pathogenesis of various parasitic infections. To investigate the biochemical characteristics and roles in the pathogenesis/host-invasion of cysteine peptidases, a cDNA sequence encoding for a cathepsin L-Iike cysteine protease (TpCP) was cloned and identified from-the T. pisiformis metacestodes. This sequence was 1220 bp in its length, which included a 1017 bp open reading frame encoding a 339 amino acid peptide. Multiple sequence alignments revealed a 28.9-88.5% similarity with cathepsin L-like cysteine proteases from other helminth parasites and mammals. The recombinant TpCP expressed in Escherichia coli did. not show the proteolytic activity by zymography gel assay. However, the TpCP expressed in Pichiapastoris had typical biochemical activities that could hydrolyze rabbit immunoglob-; ulin G, bovine serum albumin and flbronectin. Substrate studies indicated pronounced cleavage of Z-Phe-Arg-AMC. This activity was sensitive to cysteine protease inhibitor E-64 and immunohistochemistry results also indicated that TpCP was distributed as an intense positive reaction in the bladder wall. Our results gave us insights into future studies of TpCP's roles in the infection.

分类号: S85

  • 相关文献

[1]Identification and functional characterization of alpha-enolase from Taenia pisiformis metacestode. Zhang, Shaohua,Guo, Aijiang,Zhu, Xueliang,You, Yanan,Hou, Junling,Luo, Xuenong,Cai, Xuepeng,Wang, Qiuxia. 2015

[2]In situ stabilization remediation of cadmium (Cd) and lead (Pb) co-contaminated paddy soil using bentonite. Sun, Yuebing,Li, Ye,Xu, Yingming,Liang, Xuefeng,Wang, Lin,Sun, Yuebing,Xu, Yingming,Liang, Xuefeng,Wang, Lin. 2015

[3]Cloning and characterization of a gene encoding cysteine proteases from senescent leaves of Gossypium hirsutum. Shen, FF,Yu, SX,Han, XL,Fan, SL.

[4]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[5]Isolation and characterization of a cDNA encoding a papain-like cysteine protease from alfalfa. Yan, Longfeng,Han, Jianguo,Sun, Yan,Yan, Longfeng,Yang, Qingchuan,Kang, Junmei,Liu, Zhipeng,Wu, Mingsheng.

[6]Oral delivery of Bacillus subtilis spores expressing cysteine protease of Clonorchis sinensis to grass carp (Ctenopharyngodon idellus): Induces immune responses and has no damage on liver and intestine function. Tang, Zeli,Sun, Hengchang,Chen, TingJin,Lin, Zhipeng,Jiang, Hongye,Zhou, Xinyi,Ren, Pengli,Yu, Jinyun,Li, Xuerong,Xu, Jin,Huang, Yan,Yu, Xinbing,Tang, Zeli,Sun, Hengchang,Chen, TingJin,Lin, Zhipeng,Jiang, Hongye,Zhou, Xinyi,Ren, Pengli,Yu, Jinyun,Li, Xuerong,Xu, Jin,Huang, Yan,Yu, Xinbing,Tang, Zeli,Sun, Hengchang,Chen, TingJin,Lin, Zhipeng,Jiang, Hongye,Zhou, Xinyi,Ren, Pengli,Yu, Jinyun,Li, Xuerong,Xu, Jin,Huang, Yan,Yu, Xinbing,Shi, Cunbin,Pan, Houjun,Chang, Ouqin.

[7]Identification and characterization of a cathepsin-L-like peptidase in Eimeria tenella. Liu, Renqiang,Liu, Aijun,Wang, Ming,Ma, Xueting,Cai, Jianping,Zhang, Lei,Wang, Ming.

[8]Molecular characterization, immune response against white spot syndrome virus infection of peroxiredoxin 4 in Fenneropenaeus chinensis and its antioxidant activity. Zhang, Qingli,Huang, Jie,Liu, Shuang,Liu, Qinghui,Liang, Gaofeng,Li, Fuhua,Wei, Jiankai,Xiang, Jianhai. 2014

[9]Cloning, expression analysis, and antibacterial propertiesof three serum amyloid A in common carp (Cyprinus carpio). Yu, Juhua,Tang, Yongkai,Li, Jianlin,Li, Hongxia,Yu, Fan,Yu, Juhua,Yu, Wenjuan,He, Feng,Mao, Shuntao,Fu, Chunjie.

[10]Characterization of a root-specific beta-thioglucoside glucohydrolase gene in Carica papaya and its recombinant protein expressed in Pichia pastoris. Wang, Meng,Li, Dingqin,Sun, Xuepiao,Nong, Han,Zhang, Jiaming,Zhu, Yun Judy,Nong, Han. 2009

[11]A conjugate protein containing HIV TAT, ISG20, and a PRRSV polymerase binding inhibits PRRSV replication and may be a novel therapeutic platform. Liu, Ke,Li, Yuming,Huan, Beili,Shao, Donghua,Wei, Jianchao,Qiu, Yafeng,Li, Beibei,Tong, Guangzhi,Ma, Zhiyong,Zhou, Bin,Wang, Feifei,Qian, Yingjuan,Jung, Yong-Sam,Miao, Denian.

[12]Acyl-CoA oxidase 1 is involved in gamma-decalactone release from peach (Prunus persica) fruit. Zhang, Liping,Li, Haiyan,Gao, Ling,Qi, Yujie,Fu, Wanyi,Li, Xiongwei,Zhou, Xiang,Gao, Zhongshan,Jia, Huijuan,Li, Xiongwei,Gao, Qikang.

[13]Identification and characterization of a novel antibacterial peptide, avian beta-defensin 2 from ducks (Retracted article. See vol. 51, pg. 273, 2013). Wang, Ruiqin,Liao, Wenyan,Han, Zongxi,Liu, Shengwang,Ma, Deying,Wang, Ruiqin,Liao, Wenyan. 2009

[14]BQP35 is a novel member of the intrinsically unstructured protein (IUP) family which is a potential antigen for the sero-diagnosis of Babesia sp BQ1 (Lintan) infection. Guan, Guiquan,Liu, Junlong,Ma, Miling,Liu, Aihong,Niu, Qingli,Li, Youquan,Ren, Qiaoyun,Luo, Jianxun,Yin, Hong,Guan, Guiquan,Moreau, Emmanuelle,Chauvin, Alain,Rogniaux, Helene,Moreau, Emmanuelle. 2012

[15]Efficient expression and characterization of a cold-active endo-1, 4-beta-glucanase from Citrobacter farmeri by co-expression of Myxococcus xanthus protein. Bai, Xi,Yuan, Xianjun,Li, Junfeng,Shao, Tao,Bai, Xi,Wen, Aiyou,Bai, Yunfeng. 2016

[16]Evaluation of recombinant proteins of Haemophilus parasuis strain SH0165 as vaccine candidates in a mouse model. Yuan, Fangyan,Tian, Yongxiang,Yuan, Fangyan,Fu, Shulin,Hu, Jin,Li, Jinquan,Chang, Haitao,Hu, Linlin,Chen, Huanchun,Bei, Weicheng,Tian, Yongxiang. 2012

[17]Development and application of G(1)-ELISA for detection of antibodies against bovine ephemeral fever virus. Zheng, Fu-ying,Lin, Guo-zhen,Qiu, Chang-qing,Zhou, Ji-zhang,Cao, Xiao-an,Gong, Xiao-wei. 2009

[18]The epitope structure of Citrus tristeza virus coat protein mapped by recombinant proteins and monoclonal antibodies. Wu, Guan-Wei,Wang, Guo-Ping,Hong, Ni,Tang, Min,Wang, Guo-Ping,Wang, Cai-Xia,Liu, Yong,Yang, Fan,Hong, Ni,Wang, Cai-Xia,Liu, Yong.

[19]Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 and chicken IFN-gamma or IL-2 against coccidiosis in chickens. Song, Xiaokai,Huang, Xinmei,Yan, Ruofeng,Xu, Lixin,Li, Xiangrui,Huang, Xinmei.

[20]Molecular characterization and functional analysis of a peroxiredoxin 1 cDNA from golden pompano (Trachinotus ovatus). Wang, Long,Guo, Huayang,Zhang, Nan,Ma, Zhenhua,Jiang, Shigui,Zhang, Dianchang,Wang, Long.

作者其他论文 更多>>