Different toxicity of the novel Bacillus thuringiensis (Bacillales: Bacillaceae) strain LLP29 against Aedes albopictus and Culex quinquefasciatus (Diptera: Culicidae)

文献类型： 外文期刊

第一作者： Zhang, Lingling

作者： Zhang, Lingling;Tang, Baozhen;Huang, Zhipeng;Liu, Zhaoxia;Huang, Tianpei;Guan, Xiong;Huang, Enjiong;Gelbic, Ivan;Xu, Lei

作者机构：

关键词： Aedes albopictus;Bacillus thuringiensis;ELISA;Immunohistochemistry;Receptor binding

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Bacillus thuringiensis (Bt) (Berliner) strain LLP29 produces a crystal protein Cyt1Aa6 toxic to mosquito vectors of human diseases. However, the susceptibility of Culex quinquefasciatus (Say) in the current study was 8.25 times higher than that of Aedes albopictus (Skuse) with this single protein Cyt1Aa6 purified from LLP29. To understand the mechanism of the novel mosquitocidal protein, the binding characteristic of brush border membrane vesicles from the two tested mosquitoes was investigated. Enzyme-linked immunosorbent assay showed that Cyt1Aa6 bound to the two mosquitoes' brush border membrane vesicles. However, the titer of Ae. albopictus was a little higher than that of Cx. quinquefasciatus, with 3.21 and 2.91, respectively. Ligand Western blot analysis showed Cyt1Aa6 toxin specifically bound to the same three proteins (i.e., 68, 54, and 26 kDa) in the two mosquitoes, but one another protein, approximately to 37 kDa, could just be detected in Cx. quinquefasciatus. However, little difference was found in the test of immunohistochemistry. Cyt1Aa6 was detected in the midguts of both mosquitoes with histopathological changes. It would of great importance to the knowledge of the novel toxin against to Cx. quinquefasciatus and Ae. albopictus.

分类号： Q969.9

  • 相关文献

[1]Development of Bt Rice and Bt Maize in China and Their Efficacy in Target Pest Control. Liu, Qingsong,Peng, Yufa,Li, Yunhe,Hallerman, Eric. 2016

[2]Construction of an Immunized Rabbit Phage Display Library for Selecting High Activity against Bacillus thuringiensis Cryl F Toxin Single-Chain Antibodies. Xu, Chongxin,Zhang, Cunzheng,Zhong, Jianfeng,Zhang, Xiao,Liu, Yuan,Liu, Xianjin,Hu, Hui,Luo, Shimin,Liu, Xiaoqin.

[3]Epidemiology and vector efficiency during a dengue fever outbreak in Cixi, Zhejiang Province, China. Yang, Tianci,Fu, Guiming,Ding, Gangqiang,Lu, Liang,Liu, Qiyong,Zhong, Shi,Xu, Rong,Zhu, Guangfeng,Shi, Nanfeng,Fan, Feilong. 2009

[4]Detecting Splicing Variants of FOXP2 and its Protein Expression in Chicken Brain. Wang, Xi,Hamid, Mohammod Abdul,Zhao, Xingbo,Wang, Xi,Li, Hongxia. 2012

[5]Expression pattern and tissue localization of the class B scavenger receptor BmSCRBQ4 in Bombyx mori. Dong, Zhan-Peng,Huang, Ping,Liao, Peng-Fei,Liu, Min,Dai, Fang-Yin,Pan, Min-Hui,Lu, Cheng,Chai, Chun-Li,Dai, Fang-Yin,Pan, Min-Hui,Lu, Cheng,Wang, Wei,Lu, Cheng. 2015

[6]Immunolocalization of estrogen receptor alpha in Neomysis japonica oocytes and follicle cells during ovarian development. Yang, Xiaozhen,Zhao, Liulan,Zhao, Zhanzhong,Hu, Bing,Wang, Chun,Yang, Zhigang,Cheng, Yongxu,Zhao, Zhanzhong. 2012

[7]Detection methods of Cyprinid herpesvirus 2 infection in silver crucian carp (Carassius auratus gibelio) via a pORF72 monoclonal antibody. Kong, S. Y.,Jiang, Y. S.,Lu, J. F.,Xu, D.,Lu, L. Q.,Wang, Q..

[8]Expression pattern analysis of odorant-binding proteins in the pea aphid Acyrthosiphon pisum. Riviello, Lea,Falabella, Patrizia,Bruno, Daniele,Grimaldi, Annalisa,Congiu, Terenzio,Sun, Yu Feng. 2015

[9]Expression and Prognostic Values of Id-1 and Id-3 in Gastric Adenocarcinoma. Yang, Hai-Yan,Liu, Lian-Xin,Jiang, Hong-Chi,Liu, Hao-Ling,Zhu, Hong,Liu, Gai-Yun,Meng, Qing-Wen,Qu, Lian-Dong. 2011

[10]Wnt5a as a Predictor in Poor Clinical Outcome of Patients and a Mediator in Chemoresistance of Ovarian Cancer. Peng, Chunjie,Zhang, Xiaolei,Yu, Hongli,Zheng, Jianhua,Wu, Donglai. 2011

[11]Characterization and application of monoclonal antibodies against Mycoplasma hyorhinis pyruvate dehydrogenase E1 complex subunit alpha. Chen, Dongjie,Wei, Yanwu,Huang, Liping,Wang, Yiping,Du, Wenjuan,Sun, Jianhui,Wu, Hongli,Feng, Li,Liu, Changming.

[12]Molecular characterization and tissue localization of sensory neuron membrane protein from Chinese honey bee, Apis cerana cerana (Hymenoptera: Apidae). Hu, Ying-Ying,Xu, Shu-Fa,Wubie, Abebe Jenberie,Li, Wei,Huang, Jia-Xing,Guo, Zhan-Bao,Zhou, Ting,Wubie, Abebe Jenberie. 2013

[13]Expression and prognostic value of ING3 in human primary hepatocellular carcinoma. Yang, Hai-Yan,Tian, Lan-Tian,Song, Rui-Peng,Song, Xuan,Yin, Da-Long,Liang, Ying-Jian,Jiang, Hong-Chi,Liu, Lian-Xin,Liu, Hao-Ling,Qu, Lian-Dong,Liu, Jia-Ren.

[14]Characterization of a monoclonal antibody specific for human peripheral myelin protein 22 and its use in immunohistochemical studies of the fetal and adult nervous system. Gregson, Norman A.,Zhang, Gaiping,Pritchard, Jane,Wang, Aiping,Sanvito, Lara,Hayday, Adrian C.,Hughes, Richard A. C.. 2007

[15]Histochemical and immunohistochemical identification of laticifer cells in callus cultures derived from anthers of Hevea brasiliensis. Tan, Deguan,Sun, Xuepiao,Zhang, Jiaming. 2011

[16]Characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker Larimichthys crocea. Zhang, Dong Ling,Lv, Chang Huan,Wang, Zhi Yong,Yu, Da Hui.

[17]Susceptibility of farmed juvenile giant grouper Epinephelus lanceolatus to a newly isolated grouper iridovirus (genus Ranavirus). Peng, Chao,Ma, Hongling,Su, Youlu,Wen, Weigeng,Feng, Juan,Guo, Zhixun,Qiu, Lihua,Peng, Chao.

[18]Polyclonal antibodies to grain gliadins and their application in wheat quality prediction. Li, Q. Y.,Song, X. Y.,Zhang, E. Y.,Pei, Y. H.,Li, Q. Y.,Ji, K. M.,Yan, Y. M.. 2008

[19]Quantification of toxins in a Cry1Ac+CpTI cotton cultivar and its potential effects on the honey bee Apis mellifera L.. Peng Han,Chang-Ying Niu,Chao-Liang Lei,Jin-Jie Cui,Nicolas Desneux. 2010

[20]Serological Survey of Canine Leptospirosis in Southern China. Liu, Mingchao,Liu, Jianzhu,Wang, Lin,Wang, Zhenyong,Wang, Shujing,Yang, Dubao,Chai, Tongjie,Shi, Dayou,Guo, Shining,Liao, Shenquan,Sun, Mingfei. 2012

作者其他论文 更多>>

微信小程序