Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry

文献类型： 外文期刊

第一作者： Song, Suquan

作者： Song, Suquan;Ediage, Emmanuel Njumbe;De Saeger, Sarah;Song, Suquan;Wu, Aibo

作者机构：

关键词： Biomarker;LC-MS/MS;Mycotoxins;Pig urine;Salting-out liquid-liquid extraction

期刊名称：JOURNAL OF CHROMATOGRAPHY A （ 影响因子：4.759； 五年影响因子：4.302 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Direct determination of urinary mycotoxins is a better approach to assess individual's exposure than the indirect estimation from average dietary intakes. In this study, a new analytical method was developed and validated for simultaneous analysis of aflatoxin B_1, deoxynivalenol, fumonisin B_1, ochratoxin A, zearalenone and T2 toxin and their metabolites in pig urine. In total 12 analytes were selected. A salting-out assisted liquid-liquid extraction procedure was used for sample preparation. High performance liquid chromatography/tandem mass spectrometry was used for the separation and detection of all the analytes. The extraction recoveries were in a range of 70-108%, with the intra-day relative standard deviation and inter-day relative standard deviation lower than 25% for most of the compounds at 3 different concentration levels. Meanwhile the method bias for all the analytes did not exceed 20%. The limits of quantification ranged from 0.07ngmL~(-1) for ochratoxin A to 3.3ngmL~(-1) for deoxynivalenol. Matrix effect was evaluated in this study and matrix-matched calibration was used for quantification. The developed method was also validated for human urine as an extension of its application. Finally, the developed method was applied in a pilot study to analyze 28 pig urine samples. Deoxynivalenol, aflatoxin B_1, fumonisin B_1 and ochratoxin A were detected in these samples.

分类号： O65

  • 相关文献

[1]A simple sample pretreatment method for multi-mycotoxin determination in eggs by liquid chromatography tandem mass spectrometry. Zhu, Runyue,Zhao, Zhiyong,Yan, Liping,Song, Suquan,Zhao, Zhiyong,Wang, jianhua,Bai, Bing,Wu, Aibo,Song, Suquan,Wu, Aibo,Zhu, Runyue.

[2]Simultaneous determination of carbamate insecticides and mycotoxins in cereals by reversed phase liquid chromatography tandem mass spectrometry using a quick, easy, cheap, effective, rugged and safe extraction procedure. Zhang, Jin-Ming,Lu, Yao-Bin,Wu, Yin-Liang.

[3]Fusarium Populations on Chinese Barley Show a Dramatic Gradient in Mycotoxin Profiles. van der Lee, T.,Waalwijk, C.,Yang, L.,Yang, X.,Yu, D..

[4]Nonylphenol Toxicity Evaluation and Discovery of Biomarkers in Rat Urine by a Metabolomics Strategy through HPLC-QTOF-MS. Zhang, Yan-Xin,Yang, Xin,Zou, Pan,Du, Peng-Fei,Wang, Jing,Jin, Fen,Jin, Mao-Jun,She, Yong-Xin,Du, Peng-Fei,Wang, Jing,Jin, Fen,Jin, Mao-Jun,She, Yong-Xin. 2016

[5]Evaluating environmental impact of STPs situated on streams in the Czech Republic: An integrated approach to biomonitoring the aquatic environment. Li, Zhi-Hua,Zlabek, Vladimir,Turek, Jan,Velisek, Josef,Kolarova, Jitka,Sudova, Eliska,Berankova, Petra,Randak, Tomas,Pulkrabova, Jana,Hradkova, Petra,Hajslova, Jana,Li, Zhi-Hua. 2011

[6]Comparative responses in rare minnow exposed to 17 beta-estradiol during different life stages. Liao, T.,Guo, Q. L.,Xu, Y.,Liao, T.,Cheng, W.,Jin, S. W.. 2009

[7]Kinetic characters and resistance to inhibition of crude and purified brain acetylcholinesterase of three freshwater fishes by organophosphates. Li, SN,Xie, XC,Zhu, GN,Tan, YJ.

[8]Measuring the damage of heavy metal cadmium in rice seedlings by SRAP analysis combined with physiological and biochemical parameters. Zhang, Xiaoqin,Chen, Huinan,Lu, Wenyi,Pan, Jiangjie,Qian, Qian,Xue, Dawei,Jiang, Hua,Qian, Qian.

[9]Feces and liver tissue metabonomics studies on the regulatory effect of aspirin eugenol eater in hyperlipidemic rats. Yang, Yajun,Li, Jianyong. 2017

[10]Characterization of the microbial community in different types of Daqu samples as revealed by 16S rRNA and 26S rRNA gene clone libraries. Zheng, Xiao-Wei,Yan, Zheng,Han, Bei-Zhong,Zheng, Xiao-Wei,Nout, M. J. Robert,Zwietering, Marcel H.,Smid, Eddy J.,Yan, Zheng,Boekhout, Teun.

[11]Prohibitin-2 promotes hepatocellular carcinoma malignancy progression in hypoxia based on a label-free quantitative proteomics strategy. Cheng, Jun,Wu, Jian,Wu, Liming,Xie, Haiyang,Zheng, Shusen,Zhou, Lin,Cheng, Jun,Gao, Feng,Xing, Chunyang,Lv, Zhen,Wu, Liming,Ye, Sunyi,Xie, Haiyang,Zheng, Shusen,Zhou, Lin,Gao, Feng,Chen, Xiang,Xu, Wei,Xie, Qing.

[12]The application of proteomics in different aspects of hepatocellular carcinoma research. Xing, Xiaohua,Liang, Dong,Huang, Yao,Zeng, Yongyi,Liu, Xiaolong,Liu, Jingfeng,Liang, Dong,Xing, Xiaohua,Huang, Yao,Zeng, Yongyi,Liu, Xiaolong,Liu, Jingfeng,Huang, Yao,Zeng, Yongyi,Liu, Jingfeng,Han, Xiao.

[13]Quantitative Analysis of 10 Mycotoxins in Wheat Flour by Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry with a Modified QuEChERS Strategy. Zhou, Qingxin,Li, Fenghua,Chen, Leilei,Jiang, Dafeng,Li, Fenghua,Jiang, Dafeng. 2016

[14]Isolation and Characterization of an ERF Transcription Factor Gene from Cotton (Gossypium barbadense L.). Xianpeng Meng,Fuguang Li,Chuanliang Liu,Chaojun Zhang,Zhixia Wu,Yajuan Chen.

[15]Determination of 16 Mycotoxins in Maize by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry. Li, Xia,Liu, Bin,Wang, Fengen,Ma, Xinfeng,Li, Zengmei,Guo, Dongliang,Wang, Yutao,Deng, Ligang,Zhang, Shuqiu,Wan, Fachun. 2018

[16]The Mycotoxin Zearalenone Hinders Candida albicans Biofilm Formation and Hyphal Morphogenesis. Rajasekharan, Satish Kumar,Lee, Jin-Hyung,Lee, Jintae,Zhao, Yueju. 2018

[17]Simultaneous Detection of 21 Kinds of Mycotoxins and Their Metabolites in Animal Plasma with Impurity Adsorption Purification Followed by Liquid Chromatography-Tandem Mass Spectrometry. Wang Rui-Guo,Su Xiao-Ou,Wang Pei-Long,Zhang Wei,Xue Yan,Li Yu. 2017

[18]Transformation of deoxynivalenol and its acetylated derivatives in Chinese steamed bread making, as affected by pH, yeast, and steaming time. Wang, Bujun.

[19]An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles. Liu, Na,Tan, Yanglan,Wang, Hui,Wu, Aibo,Nie, Dongxia,Zhao, Zhiyong,Liao, Yucai,Sun, Changpo.

[20]Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray. Hu, Weihua,He, Guangli,Zheng, Xinting,Li, Chang Ming,Hu, Weihua,He, Guangli,Zheng, Xinting,Li, Chang Ming,Li, Xin,Zhang, Zhaowei,Li, Peiwu.

作者其他论文 更多>>

微信小程序