Development of a new duplex real-time polymerase chain reaction assay for detection of dicer in G. gallus

文献类型： 外文期刊

第一作者： Ji Xiaolin

作者： Ji Xiaolin;Wang, Qi;Gao, Yulong;Wang, Yongqiang;Qin, Liting;Qi, Xiaole;Gao, Honglei;Wang, Xiaomei

作者机构：

关键词： ALV-J;Dicer;Duplex qRT-PCR

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recently, there has been a growing body of evidence showing that cellular microRNAs (miRNAs) are involved in virus-host interactions. Numerous studies have focused on analyses of the expression profiles of cellular miRNAs, but the expression patterns of Dicer, which is responsible for the generation of miRNAs, have only rarely been explored in Gallus gallus. We developed a duplex realtime reverse transcriptase polymerase chain reaction (RTPCR) assay for the relative quantification of the mRNAs of Dicer and β-actin in G. gallus. To apply this method, the expression of Dicer in avian cells after infection with avian leukosis virus subgroup J (ALV-J) was detected using our established duplex real-time RT-PCR. The duplex realtime RT-PCR assay is sufficiently sensitive, specific, accurate, reproducible, and cost-effective for the detection of Dicer in G. gallus. Furthermore, this study, for the first time, demonstrated that ALV-J can induce differential expression of Dicer mRNA in the ALV-J-infected cells.

分类号： Q93

  • 相关文献

[1]Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae. Guo, Wen-Chao,Fu, Kai-Yun,Li, Guo-Qing,Guo, Wen-Chao,Yang, Shuai,Li, Xiao-Xu.

[2]Endogenous Retrovirus ev21 Dose Not Recombine with ALV-J and Induces the Expression of ISGs in the Host. Feng, Min,Tan, Yan,Li, Yuanfang,Xie, Tingting,Li, Hongmei,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Yuanfang,Xie, Tingting,Li, Hongmei,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Yuanfang,Xie, Tingting,Li, Hongmei,Zhang, Xiquan,Dai, Manman,Shi, Meiqing. 2016

[3]GADD45 beta, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens. Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Yan, Zhuanqiang,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Zhang, Xinheng,Li, Xinjian,Lin, Wencheng,Dai, Zhenkai,Yan, Yiming,Lu, Piaopiao,Chen, Weiguo,Chen, Feng,Ma, Jingyun,Xie, Qingmei,Lin, Wencheng,Chen, Weiguo,Chen, Feng,Xie, Qingmei,Zhang, Huanmin. 2016

[4]Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo. Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Zhenhui,Zhang, Xiquan,Dai, Manman,Shi, Meiqing. 2016

[5]NHE1 gene associated with avian leukosis virus subgroup J infection in chicken. Chen, Biao,Pan, Weiling,Zhang, Liangyu,Liu, Jing,Ouyang, Hongjia,Nie, Qinghua,Zhang, Xiquan,Chen, Biao,Pan, Weiling,Zhang, Liangyu,Liu, Jing,Ouyang, Hongjia,Nie, Qinghua,Zhang, Xiquan,Chen, Biao,Pan, Weiling,Zhang, Liangyu,Liu, Jing,Ouyang, Hongjia,Nie, Qinghua,Zhang, Xiquan.

[6]Inhibition of avian leukosis virus subgroup J replication by miRNA targeted against env. Wang, Wei,Zhang, Zai-Ping,Tian, Jin,Xiao, Zhi-Guang,Meng, Qing-Wen.

[7]Differential expression of microRNAs in avian leukosis virus subgroup J-induced tumors. Wang, Qi,Gao, Yulong,Ji, Xiaolin,Qi, Xiaole,Qin, Liting,Gao, Honglei,Wang, Yongqiang,Wang, Xiaomei. 2013

[8]An Evaluation of the Infection Status and Source of Subgroup J Avian Leukosis Virus in Cloned Free-Range Layers. Zhang Pei-pei,Liu Shao-qiong,Wang Jian,Wang Bo,Zhao Cheng-di,Sun Shu-hong,Zhang Yong-guang. 2013

[9]Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo. Gao, Yanni,Liu, Yongzhen,Guan, Xiaolu,Li, Xiaofei,Yun, Bingling,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Cui, Hongyu,Liu, Changjun,Zhang, Yanping,Wang, Xiaomei,Gao, Yulong,Wang, Xiaomei.

[10]ALV-J infection induces chicken monocyte death accompanied with the production of IL-1 beta and IL-18. Dai, Manman,Liao, Ming,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Feng, Min,Xie, Tingting,Li, Yuanfang,Ruan, Zhuohao,Zhang, Xiquan,Shi, Meiqing. 2017

[11]Avian leukosis virus subgroup J induces VEGF expression via NF-kappa B/PI3K-dependent IL-6 production. Gao, Yanni,Zhang, Yao,Guan, Xiaolu,Liu, Yongzhen,Qi, Xiaole,Wang, Yongqiang,Liu, Changjun,Zhang, Yanping,Gao, Honglei,Wang, Xiaomei,Gao, Yulong,Yao, Yongxiu,Nair, Venugopal,Yao, Yongxiu,Nair, Venugopal. 2016

[12]Genetic diversity and phylogenetic analysis of glycoprotein GP85 of ALV-J isolates from Mainland China between 1999 and 2010: Coexistence of two extremely different subgroups in layers. Pan, Wei,Gao, Yulong,Qin, Litin,Ni, Wei,Liu, Zaisi,Yun, Binglin,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei.

[13]Temporal changes of microRNA gga-let-7b and gga-let-7i expression in chickens challenged with subgroup J avian leukosis virus. Ji, Jun,Ji, Jun,Shang, Huiqin,Li, Hongxin,Ma, Jingyun,Bi, Yingzuo,Xie, Qingmei,Ji, Jun,Shang, Huiqin,Li, Hongxin,Ma, Jingyun,Bi, Yingzuo,Xie, Qingmei,Zhang, Huanmin,Ma, Jingyun,Bi, Yingzuo,Xie, Qingmei,Xie, Qingmei.

[14]ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages. Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Feng, Min,Tan, Yan,Li, Zhenhui,Zhang, Xiquan,Dai, Manman,Cao, Weisheng,Shi, Meiqing.

[15]Discovery of novel long non-coding RNAs induced by subgroup J avian leukosis virus infection in chicken. Qiu, Lingling,Li, Zhiteng,Chang, Guobin,Bi, Yulin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

作者其他论文 更多>>

微信小程序