文献类型: 外文期刊
第一作者: An, Tong-Qing
作者: An, Tong-Qing;Zhang, Yi;Tian, Zhi-Jun;Leng, Chao-Liang;Yang, Yong-Qian;Peng, Jin-Mei;Tong, Guang-Zhi;Tong, Guang-Zhi
作者机构:
关键词: Tandem repeat;Isocaudamer;short peptide;PRRSV
期刊名称:PROTEIN AND PEPTIDE LETTERS ( 影响因子:1.89; 五年影响因子:1.528 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: An improved isocaudamer tandem repeat strategy for the production of short functional peptide was demonstrated in the study. The coding sequence of short peptide was codon optimized, and two isocaudamers were induced into the end of coding sequence. By re-cutting with isocaudamers and re-ligating, the coding sequence of short peptide in the expression vector was increased in a multiple manner (21, 22, 23, 24, 25 .......). In the present study, an 8 amino-acidresidue peptide of porcine reproductive and respiratory syndrome virus (PRRSV) was effectively expressed in 8 copies and 16 copies by this approach, then the proteins in 8 copies and 16 copies were used to generate antibody against this epitope in rabbits. The results showed that PRRSVs were well recognized by the antibody in indirect immunofluorescence assay. The.technology using isocaudamer to insert multiple tandem repeats in the vector provides an important approach for the studies of small molecule peptides.
分类号: Q51
- 相关文献
作者其他论文 更多>>
-
Acid-regulated the cascade aggregation of mixed-charge ultra-small iron oxide nanoparticles to selectively amplify the imaging signal of the tumor
作者:Wang, Qingzhen;Wang, Mei;Wang, Qingzhen;Liu, Tao;Peng, Yufeng;Bai, Ru;Liu, Qiaolin;Li, Shengmin;Li, Zhenzhen;Zhou, Huige;Shi, Kejian;Li, Shengmin;Zhang, Yi;Zhang, Jiakun;Zhou, Huige;Wang, Qingzhen
关键词:Ultra-small iron oxide; PH-responsiveness; Self-assembly; MRI; Tumor fluorescence imaging
-
The effects of different extraction methods on the structure and antioxidant properties of Bletilla striata polysaccharide
作者:Han, Xue;Liu, Hai;Song, Zhiqin;Yang, Lili;Liu, Xiao;Yang, Lin;Wu, Mingkai;Han, Xue;Liu, Hai;Song, Zhiqin;Yang, Lili;Liu, Xiao;Yang, Lin;Wu, Mingkai;Zhang, Yeshan;Zhang, Yi;Tan, Longyan
关键词:Antioxidant properties;
Bletilla striata polysaccharide; extraction methods; polysaccharide structure -
Transcriptomic Profiling Highlights Metabolic and Biosynthetic Pathways Involved in In Vitro Flowering in Anoectochilus roxburghii (Wall.) Lindl.
作者:Yu, Shuisheng;Liu, Julian;Cai, Chenchen;Zhang, Yi;Fu, Shuangbin;Yang, Yanping;Zhou, Zhuang;Ying, Zhen;Fu, Shuangbin;Yang, Yanping;Ying, Zhen
关键词:flower; RNA-seq; metabolic pathways; biosynthetic pathways; DNA replication
-
Immunometabolism of Liver Xenotransplantation and Prospective Solutions
作者:Deng, Shoulong;Qin, Chuan;Deng, Shoulong;Qin, Chuan;Zhang, Yi;Shen, Shasha;Li, Chongyang
关键词:antigens; immunometabolism; liver; Treg; xenograft rejection
-
Complete Genomic Landscape Reveals Hidden Evolutionary History and Selection Signature in Asian Water Buffaloes (Bubalus bubalis)
作者:Si, Jingfang;Dai, Dongmei;Dhakal, Aashish;Omar, Abdullah Ibne;Zhang, Yi;Gorkhali, Neena Amatya;Sapkota, Saroj;Wang, Mingshan;Wang, Sheng;Wu, Dong-Dong;Kadel, Ram Chandra;Sadaula, Amir;Faruque, Md Omar;Sari, Eka Meutia;Ashari, Hidayat;Dagong, Muhammad Ihsan Andi;Yindee, Marnoch;Rushdi, Hossam E.;Amin, Ahmed;Radwan, Mohamed A.;Elregalaty, Hussein;Pham, Lan Doan;Hulugalla, W. M. M. P.;Silva, G. L. L. Pradeepa;Zheng, Wei;Mansoor, Shahid;Ali, Muhammad Basil;Mansoor, Shahid;Vahidi, Farhad;Al-Bayatti, Sahar Ahmed;Pauciullo, Alfredo;Lenstra, Johannes A.;Barker, J. Stuart F.;Fang, Lingzhao;Han, Jianlin
关键词:buffalo;
CSN2 ;MC1R ; population genomics; selection; single-cell RNA-seq -
Unravelling the gene regulatory network linking red leaf and red flesh traits in teinturier grape
作者:Li, Haoran;Zheng, Huan;Tao, Jianmin;Li, Haoran;Zhang, Yi;Sun, Chenxu;Huang, Liyuan;Dong, Yang;Yang, Yaxin;Li, Hui;Zheng, Huan;Tao, Jianmin;Zhang, Wen;Tao, Jianmin
关键词:Anthocyanins; Low-temperature; Grape leaf; Teinturier grape; Plant transformation
-
Major facility superfamily sugar transporter protein SsMFSST1 regulates Sporisorium scitamineum mating, pathogenicity, and sugar transport/absorption
作者:Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Zhang, Yi;He, Yongding;Zhang, Shilong;Zhou, Sisi;Shen, Wankuan;Yan, Meixin
关键词:sugarcane; Sporisorium scitamineum; Major Facilitator Superfamily (MFS) sugar transporter; sexual mating; pathogenicity