Expression of Short Peptide by an Improved Isocaudamer Tandem Repeat Strategy

文献类型: 外文期刊

第一作者: An, Tong-Qing

作者: An, Tong-Qing;Zhang, Yi;Tian, Zhi-Jun;Leng, Chao-Liang;Yang, Yong-Qian;Peng, Jin-Mei;Tong, Guang-Zhi;Tong, Guang-Zhi

作者机构:

关键词: Tandem repeat;Isocaudamer;short peptide;PRRSV

期刊名称:PROTEIN AND PEPTIDE LETTERS ( 影响因子:1.89; 五年影响因子:1.528 )

ISSN:

年卷期:

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收录情况: SCI

摘要: An improved isocaudamer tandem repeat strategy for the production of short functional peptide was demonstrated in the study. The coding sequence of short peptide was codon optimized, and two isocaudamers were induced into the end of coding sequence. By re-cutting with isocaudamers and re-ligating, the coding sequence of short peptide in the expression vector was increased in a multiple manner (21, 22, 23, 24, 25 .......). In the present study, an 8 amino-acidresidue peptide of porcine reproductive and respiratory syndrome virus (PRRSV) was effectively expressed in 8 copies and 16 copies by this approach, then the proteins in 8 copies and 16 copies were used to generate antibody against this epitope in rabbits. The results showed that PRRSVs were well recognized by the antibody in indirect immunofluorescence assay. The.technology using isocaudamer to insert multiple tandem repeats in the vector provides an important approach for the studies of small molecule peptides.

分类号: Q51

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