Collaborative Validation of an Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice Event TT51-1 Detection

文献类型： 外文期刊

第一作者： Wu, Yuhua

作者： Wu, Yuhua;Cao, Yinglong;Wu, Gang;Yang, Litao;Zhang, Dabing;Song, Guiwen;Shen, Ping

作者机构：

关键词： genetically modified rice;TT51-1;collaborative trial;quantitative real-time PCR

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, a collaborative trial of validating a real-time PCR method for the TT51-1 rice event was organized, including six participating laboratories. In this validation, serially diluted solutions from homogeneous genomic DNA of the TT51-1 event were used to construct standard curves of the TT51-1 event and phospholipase D (PLD) assays. The PCR efficiency was 95%, and the R2 coefficient was 0.99 for the TT51-1 system. The mean quantitative values for blind samples containing 0.1%, 0.5% 1%, 5%, and 10% (w/w) TT51-1 corresponded to 0.1%, 0.51%, 1.06%, 4.83%, and 9.62%, respectively, with a bias (%) ranging from -3.77% to 5.87%. The repeatability and reproducibility were all below 25% across the entire dynamic range. Furthermore, the measurement uncertainties of the quantitative results were estimated to be 0.10%, 0.20%, 0.40%, 1.76%, and 3.52% (w/w) for the tested samples. Both the LOD and LOQ were calculated to be 0.22%. This collaborative trial demonstrated that the TT51-1 method produces reliable, comparable, and reproducible results for a given sample set and can be adopted as a detection standard for testing laboratories.

分类号： R15

  • 相关文献

[1]Multiplex event-specific qualitative polymerase chain reaction for detecting three transgenic rice lines and application of a standard plasmid as a quantitative reference molecule. Wang, Xiaofu,Shen, Wenbiao,Wang, Xiaofu,Chen, Xiaoyun,Xu, Junfeng,Wang, Pengfei.

[2]DNA degradation in genetically modified rice with Cry1Ab by food processing methods: Implications for the quantification of genetically modified organisms. Xing, Fuguo,Zhang, Wei,Selvaraj, Jonathan Nimal,Liu, Yang.

[3]Degradation of Endogenous and Exogenous Genes of Genetically Modified Rice with Cry1Ab during Food Processing. Zhang, Wei,Xing, Fuguo,Selvaraj, Jonathan Nimal,Liu, Yang. 2014

[4]Bt rice expressing Cry2Aa does not cause direct detrimental effects on larvae of Chrysoperla sinica. Wang, Yuanyuan,Romeis, Joerg,Liu, Qingsong,Lin, Kejian,Chen, Xiuping,Peng, Yufa,Romeis, Joerg. 2013

[5]Willingness-to-accept and purchase genetically modified rice with high folate content in Shanxi Province, China. De Steur, H.,Gellynck, X.,Viaene, J.,Storozhenko, S.,Van Der Straeten, D.,Liqun, G.,Lambert, W.. 2010

[6]Successful detection of foreign inserts in transgenic rice TT51-1 (BT63) by RNA-sequencing combined with PCR. Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Huang, Shunmou,Wu, Gang,Li, Yunjing,Li, Jun,Wu, Yuhua,Cao, Yinglong,Zhu, Li,Li, Xiaofei,Wu, Gang.

[7]Real-time PCR method for detection of the transgenic rice event TT51-1. Wu, Gang,Wu, Yuhua,Nie, Shujing,Zhang, Li,Xiao, Ling,Cao, Yinglong,Lu, Changming.

[8]Characterization of the Transgenic Rice Event TT51-1 and Construction of a Reference Plasmid. Cao, Yinglong,Wu, Gang,Wu, Yuhua,Nie, Shujing,Zhang, Li,Lu, Changming.

[9]Endpoint Visual Detection of Three Genetically Modified Rice Events by Loop-Mediated Isothermal Amplification. Chen, Xiaoyun,Wang, Xiaofu,Zhou, Yu,Huang, Sainan,Miao, Qingmei,Zhu, Qing,Xu, Junfeng,Jin, Nuo,Huang, Sainan. 2012

[10]Development of the One-step Visual Loop-Mediated Isothermal Amplification Assay for Genetically Modified Rice Event TT51-1. Chen, Rui,Wang, Yong,Zhu, Zhu,Lan, Qingkuo,Zhao, Xin,Guo, Yongze,Wang, Yong,Wang, Qinying,Wang, Haiyang.

[11]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[12]Reference genes for quantitative real-time PCR analysis and quantitative expression of P5CS in Agropyron mongolicum under drought stress. Tian Qing-song,Du Jian-cai,Han Bing,Wang Shu-yan,Wu Zhi-juan,Li Xiao-quan,Han Bing. 2016

[13]Characterization, molecular cloning, and expression analysis of Ecsit in the spinyhead croaker, Collichthys lucidus. Song, W.,Jiang, K. J.,Zhang, F. Y.,Wang, J.,Ma, L. B.. 2016

[14]Identification and characterization of (E)-beta-caryophyllene synthase and alpha/beta-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton. Xiao, Yutao,Zhang, Wanna,Wu, Juan,Guo, Yuyuan,Zhang, Yongjun,Huang, Xinzheng,Wu, Junxiang,Koellner, Tobias G.. 2013

[15]Characterization and expression analysis of the prophenoloxidase activating factor from the mud crab Scylla paramamosain. Wang, J.,Jiang, K. J.,Zhang, F. Y.,Song, W.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.,Ma, L. B.,Wang, J.,Zhao, M.,Wei, H. Q.,Meng, Y. Y.. 2015

[16]Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis). Ma Qian,Zhuang Zhimeng,Feng Wenrong,Liu Shufang,Tang Qisheng. 2015

[17]Gene expression profiling in the thiamethoxam resistant and susceptible B-biotype sweetpotato whitefly, Bemisia tabaci. Zhou, Xu-guo,Xie, Wen,Yang, Xin,Wang, Shao-li,Wu, Qing-jun,Yang, Ni-na,Li, Ru-mei,Jiao, Xiao-guo,Pan, Hui-peng,Liu, Bai-ming,Feng, Yun-tao,Xu, Bao-yun,Zhang, You-jun. 2012

[18]Validation of Reference Genes for Quantitative Real-Time PCR during Bicolor Tepal Development in Asiatic Hybrid Lilies (Lilium spp.). Xu, Leifeng,Xu, Hua,Cao, Yuwei,Yang, Panpan,Feng, Yayan,Tang, Yuchao,Yuan, Suxia,Ming, Jun,Yang, Panpan. 2017

[19]Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon). Wang, Y.,Fu, M. J.,Zhao, C.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Wang, Y.,Fu, M. J.,Zhao, C.,Bao, W. Y.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Yang, Q. B.. 2016

[20]Molecular characterization and expression of a novel big defensin (Sb-BDef1) from ark shell, Scapharca broughtonii. Li, Meng,Zhu, Ling,Zhou, Chun-ya,Sun, Shan,Fan, Yan-jun,Zhuang, Zhi-meng,Li, Meng,Zhou, Chun-ya. 2012

作者其他论文 更多>>

微信小程序