Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus
文献类型： 外文期刊
第一作者： Li, Yongfeng
作者： Li, Yongfeng;Shen, Liang;Sun, Yuan;Yuan, Jin;Huang, Junhua;Li, Chao;Li, Su;Luo, Yuzi;Qiu, Hua-Ji
作者机构：
期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.
分类号： R37
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