Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

文献类型: 外文期刊

第一作者: Chen, Jianfei

作者: Chen, Jianfei;Liu, Xiaozhen;Shi, Da;Shi, Hongyan;Zhang, Xin;Feng, Li;Lang, Hongwu;Wang, Zhongtian

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期刊名称:ARCHIVES OF VIROLOGY ( 影响因子:2.574; 五年影响因子:2.466 )

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收录情况: SCI

摘要: Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006-2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.

分类号: R37

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