The plant cytoskeleton controls regulatory volume increase

文献类型： 外文期刊

第一作者： Liu, Qiong

作者： Liu, Qiong;Ismail, Ahmed;Chang, Xiaoli;Nick, Peter;Qiao, Fei

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关键词： Cytoskeleton;Osmotic expansion;Protoplast;Regulatory cell volume control;Vitis

期刊名称：BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES （ 影响因子：3.747； 五年影响因子：4.404 ）

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收录情况： SCI

摘要： The ability to adjust cell volume is required for the adaptation to osmotic stress. Plant protoplasts can swell within seconds in response to hypoosmotic shock suggesting that membrane material is released from internal stores. Since the stability of plant membranes depends on submembraneous actin, we asked, whether this regulatory volume control depends on the cytoskeleton. As system we used two cell lines from grapevine which differ in their osmotic tolerance and observed that the cytoskeleton responded differently in these two cell lines. To quantify the ability for regulatory volume control, we used hydraulic conductivity (. Lp) as readout and demonstrated a role of the cytoskeleton in protoplast swelling. Chelation of calcium, inhibition of calcium channels, or manipulation of membrane fluidity, did not significantly alter Lp, whereas direct manipulation of the cytoskeleton via specific chemical reagents, or indirectly, through the bacterial elicitor Harpin or activation of phospholipase D, was effective. By optochemical engineering of actin using a caged form of the phytohormone auxin we can break the symmetry of actin organisation resulting in a localised deformation of cell shape indicative of a locally increased Lp. We interpret our findings in terms of a model, where the submembraneous cytoskeleton controls the release of intracellular membrane stores during regulatory volume change.

分类号： Q73

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