Establishment of real-time TaqMan-fluorescence quantitative RT-PCR assay for detection and quantitation of ten kinds of porcine inflammation markers mRNA
文献类型: 外文期刊
第一作者: Lin, Wencheng
作者: Lin, Wencheng;Qiu, Zheng;Liu, Qinfang;Cui, Shangjin;Wang, Zhongtian
作者机构:
关键词: Cytokine;Porcine;Real-time PCR;Type I interferon;Inflammation markers
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Real-time PCR assays based on TaqMan probes for detection of porcine inflammation markers including interferon-alpha (IFN-alpha), interferon-beta (IFN-beta), retinoic acid-inducible gene 1 (RIG-1), toll-like receptor 9 (TLR-9), interferon regulatory factors (IRF-3, IRF-7), Janus kinase (JAK-1, JAK-2), signal transducers and activators of transcription (STAT-1, STAT-2) were established in this study. The results indicated that the established assays were highly specific and sensitive with a detection limit of 1.0 x 10(1) copies/mu l, and coefficient of variations was less than three percent for both inter- and intra-assay. The established assays were used to detect mRNA levels of these inflammation markers and beta-actin in swine testicle (ST) cells transfected with polyinosinic: polycytidylic acid (poly (I:C)). The results showed that the transcription levels (mRNA) of IFN-alpha, IFN-beta, RIG-1 and IRF-7 were up-regulated in ST cells transfected with poly (LC), and the transcription levels (mRNA) of TLR-9, IRF-3, JAK-1, JAK-2, STAT-1 and STAT-2 showed minimal change. The real-time PCR assays established in this study with high specificity, sensitivity and reproducibility could be used to quantify mRNA levels of porcine inflammation markers
分类号: R37
- 相关文献
作者其他论文 更多>>
-
The Generation of a H9N2 Avian Influenza Virus with HA and C3d-P29 Protein Fusions and Vaccine Development Applications
作者:Pan, Xue;Zhou, Fan;Shi, Xiaona;Liu, Qinfang;Yan, Dawei;Teng, Qiaoyang;Yuan, Chunxiu;Xu, Bangfeng;Zhang, Zhifei;Yan, Minghao;Li, Zejun
关键词:maternal-derived antibody; vaccine; hemagglutinins; complement
-
Development of an RT-qPCR Assay for the Detection of an Emerging Duck Egg-Reducing Syndrome
作者:Zhang, Zhifei;Su, Xin;Shuo, Dun;Yan, Dawei;Pan, Xue;Xu, Bangfeng;Yan, Minghao;Ren, Shuxuan;Liu, Qinfang;Yuan, Chunxiu;Teng, Qiaoyang;Li, Zejun
关键词:DERSV; RT-qPCR; TaqMan; specificity; sensitivity
-
Isolation and Characterization of H1 Subtype Swine Influenza Viruses Recently Circulating in China
作者:Yan, Minghao;Ma, Tianxin;Shi, Xiaona;Chen, Qin;Li, Luzhao;Xu, Bangfeng;Pan, Xue;Teng, Qiaoyang;Yuan, Chunxiu;Yan, Dawei;Zhang, Zhifei;Liu, Qinfang;Li, Zejun
关键词:swine influenza virus; H1 subtype; reassortant; replication; potential threat
-
Transmission routes of cluster 3 Tembusu virus in ducks and chickens
作者:Li, Luzhao;Yan, Dawei;Shuo, Dun;Shi, Xiaona;Yan, Minghao;Yuan, Chunxiu;Teng, Qiaoyang;Xu, Bangfeng;Pan, Xue;Liu, Qinfang;Li, Zejun;Li, Luzhao;Oers, Monique M. van;Pijlman, Gorben P.
关键词:chicken; duck; flavivirus; pathogenicity; Tembusu virus; transmission route
-
The Isolation and Construction of an Infectious Clone for a Duck Adenovirus Type 3 Strain
作者:Lu, Haipeng;Wu, Mi;Wang, Fenglong;Li, Zejun;Lu, Haipeng;Tang, Mei;Zhang, Zhifei;Wu, Mi;Yuan, Chunxiu;Pan, Xue;Liu, Qinfang;Teng, Qiaoyang;Xu, Bangfeng;Yan, Minghao;Yan, Dawei;Li, Zejun
关键词:duck adenovirus type 3 (DAdV-3); infectious clone; Hexon gene; biological characteristics
-
UBE2S facilitates glioblastoma progression through activation of the NF-κB pathway via attenuating K11-linked ubiquitination of AKIP1
作者:Han, Zhibin;Xu, Lin;Wang, Aowen;Gang, Zhenbo;Lin, Zhiguo;Hu, Li;Wang, Baoju;Liu, Qinfang;Liu, Hongyang;Weng, Changjiang;Liu, Qi;Yu, Shengkun;Mu, Long;Mu, Long
关键词:GBM; UBE2S; AKIP1; Deubiquitination; NF-kappa B
-
Critical role for ribonucleoside-diphosphate reductase subunit M2 in ALV-J-induced activation of Wnt/β-catenin signaling via interaction with P27
作者:Tang, Shuang;Leng, Mei;Tan, Chen;Zhu, Lin;Pang, Yanling;Zhang, Xinheng;Lin, Wencheng;Chang, Yung-Fu
关键词:ALV-J; RRM2; Wnt/beta-catenin signaling; replication