Immunoadjuvant efficacy of CpG plasmids for H9N2 avian influenza inactivated vaccine in chickens with maternal antibodies

文献类型： 外文期刊

第一作者： Pan, Xue

作者： Pan, Xue;Liu, Qinfang;Niu, Shiqi;Yan, Dawei;Teng, Qiaoyang;Li, Xuesong;Li, Zejun;de Jong, Mart C. M.;Forlenza, Maria;Beerens, Nancy;Beerens, Nancy;Li, Zejun

作者机构：

关键词： Maternal-derived antibodies (MDAs); Passively transferred antibodies (PTAs); CpG; Vaccine adjuvant

期刊名称：VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY （ 影响因子：1.8； 五年影响因子：2.0 ）

ISSN： 0165-2427

年卷期： 2023 年 259 卷

页码：

收录情况： SCI

摘要： Maternal-derived antibodies (MDAs) are one of reasons why vaccination with the H9N2 inactivated whole virus (IWV) vaccine failed in poultry. Unmethylated CpG motif-containing oligodeoxynucleotides (CpG ODN) shows great potential to overcome MDAs interference in mammals, but whether it has similar characteristics in poultry is still unknown. In the present study, different classes and various copies of CpG ODN motifs were cloned into two different plasmids (pCDNA3.1 or T vector). Immunomodulatory activities and immunoadjuvant efficacy of these CpG ODN plasmids were tested in vitro and in vivo in the presence of passively transferred antibodies (PTAs) that were used to mimic MDAs. Results showed that the T vector enriched with 30 copies of CpG-A ODN and 20 copies of CpG-B ODN (T-CpG-AB) significantly up-regulated mRNA expression of chicken-interferon-& alpha; (ch-IFN-& alpha;), chicken-interferon-& beta; (ch-IFN-& beta;) and chicken-interleukin-12 protein 40 (ch-IL-12p40). When administered as adjuvant of the H9N2 IWV vaccine, the minimal dose of T-CpG-AB plasmid was 30 & mu;g per one-day-old chicken, which could induce strong humoral immune responses in the presence of PTAs. Furthermore, T-CpG-AB plasmid-based vaccine triggered both strong humoral immune responses and cytokines expression in the presence of PTAs in chickens. Overall, our findings suggest that T-CpG-AB plasmid can be an excellent adjuvant candidate for the H9N2 IWV vaccine to overcome MDAs interference in chickens.

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