Enhance immune response to H9 AIV DNA vaccine based on polygene expression and DGL nanoparticle encapsulation

文献类型： 外文期刊

第一作者： Xu, Shangen

作者： Xu, Shangen;Jin, Zheng;Qu, Yang;Li, Jiawei;Zhang, Qihong;Zhao, Kai;Yu, Lu;Teng, Qiaoyang;Li, Xuesong;Li, Zejun

作者机构：

关键词： avian influenza; dendrimer polylysine nanoparticles; DNA nanovaccine

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 10 期

页码：

收录情况： SCI

摘要： DNA vaccination has great potential to treat or prevent avian influenza pandemics, but the technique may be limited by low immunogenicity and gene delivery in clinical testing. Here, to improve the immune efficacy of DNA vaccines against avian influ-enza, we prepared and tested the immunogenicity of 4 recombinant DNA vaccines containing 2 or 3 AIV anti-gens. The results revealed that chickens and mice immu-nized with plasmid DNA containing 3 antigens (HA gene from H9N2, and NA and HA genes from H5N1) exhibited a robust immune response than chickens and mice immunized with plasmid DNAs containing 2 anti-genic genes. Subsequently, this study used pf3H9N1SH5 as a model antigen to study the effect of dendritic polyly-sine (DGL) nanoparticles as a gene delivery system and adjuvant on antigen-specific immunity in mice models. At a ratio of 1:3 DGL/pf3H9N1SH5 (w/w), the pf3H9N1SH5/DGL NPs showed excellent physical and chemical properties, induced higher levels of HI antibod-ies, and larger CD3+/CD4+ T lymphocyte and CD3 +/CD8+ T lymphocyte population, as well as the pro-duction of cytokines, namely, interferon (IFN)-g, inter-leukin (IL)-2 compared with the naked pf3H9N1SH5. Therefore, multiantigen gene expression methods using DGL as a delivery system may have broad application prospects in gene therapy.

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