Spatial distribution and expression of intracellular and extracellular acid phosphatases of cluster roots at different developmental stages in white lupin
文献类型: 外文期刊
第一作者: Tang, Hongliang
作者: Tang, Hongliang;Li, Xiaoqing;Zu, Chao;Zhang, Fusuo;Shen, Jianbo;Tang, Hongliang;Zu, Chao
作者机构:
关键词: Acid phosphatase;Cluster roots;Fluorescent labelling;Phosphorus deficiency;White lupin
期刊名称:JOURNAL OF PLANT PHYSIOLOGY ( 影响因子:3.549; 五年影响因子:4.164 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Acid phosphatases (APases) play a key role in phosphorus (P) acquisition and recycling in plants. White lupin (Lupinus albus L) forms cluster roots (CRs) and produces large amounts of APases under P deficiency. However, the relationships between the activity of intracellular and extracellular APases (EC 3.1.3.2) and CR development are not fully understood. Here, comparative studies were conducted to examine the spatial variation pattern of APase activity during CR development using the enzyme-labelled fluorescence-97 (ELF-97) and the p-nitrophenyl phosphate methods. The activity of intracellular and extracellular APases was significantly enhanced under P deficiency in the non-CRs and CRs at different developmental stages. These two APases exhibited different spatial distribution patterns during CR development, and these distribution patterns were highly modified by P deficiency. The activity of extracellular APase increased steadily with CR development from meristematic, juvenile, mature to senescent stages under P deficiency. In comparison, P deficiency-induced increase in the activity of intracellular APase remained relatively constant during CR development. Increased activity of intracellular and extracellular APases was associated with enhanced expression of LaSAP1 encoding intracellular APase and LaSAP2 encoding extracellular APase. The expression levels of these two genes were significantly higher at transcriptional level in both mature and senescent CRs. Taken together, these findings demonstrate that both activity and gene expression of intracellular or extracellular APases exhibit a differential response pattern during CR development, depending on root types, CR developmental stages and P supply. Simultaneous in situ determination of intracellular and extracellular APase activity has proved to be an effective approach for studying spatial variation of APases during CR development. (C) 2013 Elsevier GmbH. All rights reserved.
分类号: Q94
- 相关文献
作者其他论文 更多>>
-
Polysaccharide from Scutellaria baicalensis Georgi alleviates ulcerative colitis in mice by maintaining the intestinal barrier integrity via MLC/Notch signaling
作者:Xiao, Liang;Chen, Yongpo;Li, Xiaoqing;Li, Shuwei;Tang, Wenjie;Yan, Jiayou;Wei, Xiaolan;Zhou, Mengjia;Huang, Chongbo;Diao, Hui;Li, Shuwei;Tang, Wenjie;Yan, Jiayou;Wei, Xiaolan;Zhou, Mengjia;Huang, Chongbo;Diao, Hui
关键词:ulcerative colitis; Scutellaria baicalensis Georgi; polysaccharides; intestinal barrier; notch; myosin
-
Optimising Peanut Growth: Exogenous Calcium Enhances Photosynthesis in Phosphorus-Limited Environments
作者:Shi, Qingwen;Ma, Mingzhu;Liu, Yifei;Sun, Zhiyu;Salam, Shaikh Amjad;Zhang, Siwei;Liu, Huan;Shi, Qingwen;Zhang, Fusuo;Lambers, Hans;Bai, Chunming;Bai, Chunming;Liu, Yifei;Pang, Jiayin;Siddique, Kadambot H. M.;Lambers, Hans;Liu, Yifei;Siddique, Kadambot H. M.;Liu, Yifei;Pang, Jiayin;Lambers, Hans
关键词:calcium; feedback inhibition; peanut; phosphorus; photosynthesis
-
The agricultural plastic paradox: Feeding more, harming more?
作者:Wang, Kai;Liu, Xuejun;Zhang, Fusuo;Wang, Kai;Liu, Xuejun;Zhang, Fusuo;Wang, Kai;Liu, Xuejun;Zhang, Fusuo;Chadwick, David R.;Jones, Davey L.;Yan, Changrong;Reay, Michaela;Lloyd, Charlotte;Evershed, Richard P.;Ge, Tida;Ding, Fan;Wang, Jingkuan;Qi, Ruimin;Xiao, Mouliang;Jiang, Rui;Chen, Yanling;Ma, Ji;Luo, Yongming;Zhu, Yongguan
关键词:Agroecosystem resilience; Plastic pollution; Sustainable development goals; Nanoplastic
-
Niche and interspecific association of dominant zooplankton species near the Taishan coastal area in the South China Sea
作者:He, Rui;Hu, Ren;Xiao, Bingqing;Wei, Jingxin;Wu, Peng;He, Rui;Tang, Hongliang;Tang, Hongliang;Wu, Peng
关键词:Zooplankton; Dominant species; Ecological niche; Interspecies association
-
Low ammonium and high nitrate input improves nitrogen use efficiency and growth in sugarcane through coordinated reprogramming of nitrogen and carbon metabolism
作者:Zhan, Jian;Zhou, Yifan;Yang, Linsheng;Deng, Ke;Zhang, Di;Meng, Bo;Wang, Huayang;Deng, Yan;Chen, Xinping;Zhang, Fusuo;Zhan, Jian;Zhou, Yifan;Yang, Linsheng;Deng, Ke;Zhang, Di;Meng, Bo;Wang, Huayang;Deng, Yan;Chen, Xinping;Zhan, Jian;Wang, Huayang;Deng, Yan;Chen, Xinping;Lakshmanan, Prakash;Lakshmanan, Prakash;Zhang, Fusuo
关键词:Ammonium-to-nitrate-ratio; Ammonium toxicity; Multi-omics; Nitrogen assimilation; Sugarcane
-
Antibiotics Sources, Concentrations, and Risk of Antimicrobial Resistance Selection in the Three Gorges Reservoir Basin, China
作者:Li, Shiyang;Zhang, Fusuo;Chen, Xuanjing;Chen, Xinping;Li, Shiyang;Zhang, Qi;Chen, Meijun;Strokal, Maryna;Kroeze, Carolien;Hofstra, Nynke;Chen, Meijun;Ma, Lin;Chen, Xinping
关键词:wastewater; animal manure; soil; river; model development
-
Effects of immersion with electro-activated alkaline water on gel properties, biochemicals and odor characteristics of myofibrillar proteins
作者:Lin, Yilin;Li, Xiaoqing;Wang, Chun;Yu, Yigang;Lin, Yilin;Zhang, Yehui;Huang, Lihua;Wang, Jili
关键词:Myofibrillar proteins; Alkaline water; Surimi gels; Water distribution; Microstructure; Volatile compounds