Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae

文献类型: 外文期刊

第一作者: Qi, Zemin

作者: Qi, Zemin;Zou, Yuanchao;Yue, Xingjian;Xie, Biwen;Zhao, Daxian;Gong, Quan

作者机构:

关键词: Heat shock protein 70;Botia reevesae;Ammonia-N;Aeromonas hydrophila

期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )

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收录情况: SCI

摘要: The objective of this study was to investigate the effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. After ammonia-N exposure at a constant concentration of 7.21 +/- 0.10 mg L-1 for 96 h, B. reevesae was challenged with Aeromonas hydrophila. Quantitative PCR analysis showed predominant and significant expression of HSP70 in liver, gill, skin, spleen and kidney (P < 0.05), with significantly upregulated expression of the mRNA transcript in these tissues after sublethal ammonia-N exposure and A. hydrophila challenge. Furthermore, following A. hydrophila challenge after ammonia-N exposure, HSP70 mRNA expression was significantly upregulated in kidney and gill tissues, although its expression levels were significantly lower than those detected following A. hydrophila challenge or ammonia-N exposure individually. These results indicate that B. reevesae HSP70 is involved in resistance to pathogenic bacteria. It is hypothesized that ammonia-N results in the downregulation of HSP70 mRNA in immune organs after an A. hydrophila challenge, thus lowering their resistance to pathogenic stress. (C) 2013 Published by Elsevier Ltd.

分类号: S9

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