Development and evaluation of specific PCR and LAMP assays for the rapid detection of Phytophthora melonis

文献类型： 外文期刊

第一作者： Chen, Qinghe

作者： Chen, Qinghe;Li, Benjin;Liu, Peiqing;Lan, Chengzhong;Zhan, Zhixiong;Weng, Qiyong

作者机构：

关键词： Loop-mediated isothermal amplification (LAMP);Molecular detection;Phytophthora melonis;Ras-related protein (Ypt1) gene

期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：1.907； 五年影响因子：2.022 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Phytophthora melonis is a widespread and devastating pathogen for the Cucurbitaceae family. Early and accurate detection of P. melonis is essential to control the disease in the field. To establish a simple, visual, and rapid detection system for P. melonis, we developed nested polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) systems based on the Ras-related protein (Ypt1) gene. All 36 isolates of P. melonis, from geographically distinct counties in China, yielded positive detection results on LAMP or nested PCR assays. No cross reaction was observed with other oomycetes or fungal pathogens. A sensitivity assay showed that both methods had a detection limit of 10 fg genomic DNA. We also detected P. melonis in diseased cucumber tissues and soils, and evaluated positive detection rates using LAMP, nested PCR, and conventional isolation methods. The results suggest that the LAMP assay has the greatest potential for active detection of P. melonis in regions that are at risk of contracting the disease, and for use in resource-poor settings.

分类号： S432.1

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