Establishment and biological characteristics of a jingning chicken embryonic fibroblast bank

文献类型： 外文期刊

第一作者： Bai, C.

作者： Bai, C.;Jin, D.;Li, C.;Guan, W.;Ma, Y.;Wang, D.;Li, C.

作者机构：

关键词： Biological characteristics;Fibroblasts;Genetic conservation;Jingning chicken

期刊名称：EUROPEAN JOURNAL OF HISTOCHEMISTRY （ 影响因子：3.188； 五年影响因子：2.589 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Using tissue explantation and cryopreservation biotechniques, a Jingning chicken embryonic fibroblast bank was successfully established, which includes 43 embryo samples and a stock of 178 cryovials, each one containing 3.0×10 6 cells. Most of the cells were apparently fibroblasts in their morphology, and the population doubling time (PDT) was about 48 h. The total chromosome number of a diploid cell was 78. According to karyotyping and G-banding, the diploid rate in the cell bank was 97.62±2.12%. The cells were tested for microbial contamination and found free of infections from bacteria, fungi, viruses and mycoplasms. There was no cross-contamination from other cell lines as revealed by lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzyme polymorphisms. Six fluorescent proteins were transfected into the Jingning chicken embryonic fibroblasts, and the transfection efficiencies of these genes were between 10.1 and 41.9%. All the tests showed that the quality of the cell line conforms to the quality criteria of the ATCC (American type culture collection). This work succeeded not only in preserving the genetic resources of Jingning chicken, but it also established a new protocol to preserve endangered animal breeds.

分类号： Q136

  • 相关文献

[1]Establishment and characterization of a Chinese Game chicken embryo fibroblast bank. Li, Xiangchen,Bai, Chunyu,Su, Xiaohua,Jin, Dapeng,Zhao, Qianjun,He, Xiaohong,Guan, Weijun,Ma, Yuehui,Bai, Chunyu,Jin, Dapeng,Su, Xiaohua.

[2]Establishment and biological research of the Jining Grey goat fibroblast line. Bai, Chunyu,Su, Xiaohua,Guan, Weijun,Ma, Yuehui,Bai, Chunyu,Zhang, Minghai,Wang, Dianjun. 2012

[3]Attempt at conserving the genetic resources of Hu sheep by fibroblast line cryopreservation. Li, Xiangchen,Hu, Pengfei,He, Xiaohong,Lu, Taofeng,Guan, Weijun,Ma, Yuehui.

[4]Isolation and characterization of twenty polymorphic microsatellites for Hemibarbus labeo (Cyprinidae). Lu, Cuiyun,Gu, Ying,Geng, Longwu,Cheng, Lei,Li, Chao,Tong, Guangxiang,Jiang, Haifeng,Xu, Wei,Sun, Xiaowen,Mahboob, Shahid.

[5]A first set of polymorphic microsatellite loci isolated from Rhinogobio cylindricus. Duan, Xin Bin,Chen, Da Qing,Liu, Shao Ping,Liu, Hong Yan,Xiong, Fei,Zhang, Fan Rrong,Yang, Dong,Yu, Lai Ning.

[6]Transcript characteristic of myostatin in sheep fibroblasts. Lu, Jian,Ding, Jiatong,Li, Bichun,Lu, Jian,Zhang, Xiaoning,Zhao, Fuping,Zhou, Xinlei,Zhang, Li,Wei, Caihong,Du, Lixin,Ren, Hangxing,Sheng, Xihui,Li, Shangang.

[7]Lead induces Siberian tiger fibroblast apoptosis by interfering with intracellular homeostasis. Liu, Zheng,Wang, Hui,Guo, Xuesong,Zhang, Wenxiu,Yuan, Ziao,Yuan, Hongyi,Liu, Xueting,Guan, Weijun,Zhang, Minghai. 2018

[8]Reversine Increases the Plasticity of Long-Term Cryopreserved Fibroblasts to Multipotent Progenitor Cells through Activation of Oct4. Li, Xiangchen,Guo, Yu,Yao, Yaxin,Ma, Yuehui,Liu, Changqing,Guan, Weijun,Guo, Yu,Liu, Changqing,Hua, Jinlian. 2016

[9]The effect of myostatin silencing by lentiviral-mediated RNA interference on goat fetal fibroblasts. Lu, Jian,Wei, Caihong,Zhang, Xiaoning,Xu, Lingyang,Zhang, Shifang,Liu, Jiasen,Cao, Jiaxue,Zhao, Fuping,Zhang, Li,Du, Lixin,Lu, Jian,Lu, Jian,Li, Bichun,Liu, Jiasen,Cao, Jiaxue.

[10]Establishment and Characterization of a Fibroblast Line from Duroc. Xiong, Hui,He, Xiaohong,Zhang, Wenxiu,Li, Changli,Li, Mei,Guan, Weijun,Ma, Yuehui,Xiong, Hui. 2014

[11]Overexpression of SIRT6 in Porcine Fetal Fibroblasts Attenuates Cytotoxicity and Premature Senescence Caused by D-Galactose and Tert-Butylhydroperoxide. Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Xie, Xiaoxian,Zhang, Hao,Wang, Liangliang,Zhang, Ailing,Li, Jiaqi,Gao, Ping,Xie, Shuihua.

[12]Optimization of the Transfection Efficiency of Buffalo Fetal Fibroblasts Cells Using Liposome. Deng, Tingxian,Pang, Chunying,Zhang, Yingyin,Yang, Bingzhuang,Liang, Xianwei. 2013

[13]Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan,Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan.

[14]Apoptosis Induced by Duck Reovirus p10.8 Protein in Primary Duck Embryonated Fibroblast and Vero E6 Cells. Geng, Hongwei,Zhang, Yun,Guo, Dongchun,Wang, Yu,Liu, Ming,Zhang, Yun,Liu-Partanen, Yin,Tong, Guangzhi.

[15]Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig. Hua, Z.,Liu, X.,Bi, Y.,Xiao, H.,Hua, W.,Li, L.,Zhang, L.,Ren, H.,Zheng, X.,Xu, G..

[16]Isolation and functional analysis of the pathogenicity-related gene VdPR3 from Verticillium dahliae on cotton. Zhang, Ya-Lin,Li, Zhi-Fang,Feng, Zi-Li,Feng, Hong-Jie,Zhao, Li-Hong,Shi, Yong-Qiang,Hu, Xiao-Ping,Zhu, He-Qin.

[17]Establishment of Big Bone chicken fibroblast cell bank and study of its biological characteristics. Su, Xiaohua,Jin, Dapeng,Yue, Hua,Pu, Yabin,Ma, Yuehui,Guan, Weijun,Su, Xiaohua,Li, Chunying,Yue, Hua,Jin, Dapeng. 2011

[18]Biological characterization of sheep kidney-derived mesenchymal stem cells. Ji, Meng,Bai, Chunyu,Fan, Ya'nan,Ma, Caiyun,Li, Xiangchen,Guan, Weijun,Li, Lu. 2016

[19]Sensitivity to fluopicolide of wild type isolates and biological characteristics of fluopicolide-resistant mutants in Pseudoperonospora cubensis. Wang, Wenqiao,Yan, Lei,Meng, Runjie,Zhao, Jianjiang,Zhang, Xiaofeng,Han, Xiuying,Ma, Zhiqiang. 2014

[20]Establishment and Biological Characteristic Research of Yuxizhiwei sheep Fibroblast Cell Bank. Wang, Hui,Li, Xiangchen,Li, Changli,Zhang, Wenxiu,Guan, Weijun,Ma, Yuehui,Wang, Hui. 2012

作者其他论文 更多>>

微信小程序