Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers

文献类型: 外文期刊

第一作者: Li, Xiaoli

作者: Li, Xiaoli;Xie, Jingjing;Lu, Lin;Zhang, Liyang;Zhang, Lingyan;Luo, Xugang;Li, Xiaoli;Zou, Yaxue;Wang, Qiuyue;Li, Sufen

作者机构:

关键词: Caco-2 cell;Divalent metal transporter 1;Ferroportin;Manganese;Transport

期刊名称:BIOMETALS ( 影响因子:2.949; 五年影响因子:2.978 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Two experiments were conducted to investigate the kinetics of manganese (Mn) transport in Caco-2 cell monolayers and the gene expressions of Mn transport carriers in apical (AP) and basolateral (BL) membranes. In experiment 1, the cells were treated with the medium containing 146 μmol/L of Mn (MnSO_4·H_2O). Both the uptake and transport of Mn from AP-BL or from BL-AP at different time-points were assessed to determine the optimal time for kinetics of Mn transport. The transport of Mn increased linearly with higher efficiency values in AP-BL than in BL-AP direction, however, the uptake of Mn revealed an asymptotic pattern within 120 min. In experiment 2, the kinetics of Mn transport in AP-BL was determined with media containing Mn concentrations from 0 to 2,500 μmol/L at 40 and 120 min, respectively, and mRNA levels of divalent metal transporter 1 (DMT1) and ferroportin (FPN1) were determined in Caco-2 cells treated with the medium containing 0 or 800 μmol/L of Mn for 120 min. The kinetics of Mn transport showed a carrier-mediated process when Mn concentrations were lower than 1,000 μmol/L and a linear increment when Mn concentrations exceeded 1,000 μmol/L at either 40 or 120 min. Mn treatment decreased (P < 0.01) DMT1 mRNA level and increased (P < 0.01) FPN1 mRNA level. The results from the present study suggested that Mn transport in AP-BL fit both carrier-mediated saturable and non-saturable diffusion processes, and Mn transport carriers DMT1 and FPN1 mediate the apical uptake and basolateral exit of Mn in Caco-2 cells.

分类号: Q

  • 相关文献

[1]Kinetics of iron absorption by in situ ligated small intestinal loops of broilers involved in iron transporters. Zhang, L. Y.,Liao, X. D.,Zhang, L. Y.,Lu, L.,Luo, X. G.,Zhang, L. Y..

[2]Effect of iron source on iron absorption and gene expression of iron transporters in the ligated duodenal loops of broilers. Zhang, L. Y.,Li, X. F.,Liao, X. D.,Zhang, L. Y.,Lu, L.,Luo, X. G.,Zhang, L. Y.,Zhang, L. Y.. 2017

[3]Cloning, sequencing, characterization, and expressions of divalent metal transporter one in the small intestine of broilers. Bai, S. P.,Lu, L.,Luo, X. G.,Liu, B.,Bai, S. P.,Lu, L.,Luo, X. G.,Liu, B.. 2008

[4]Effects of water stress at different deficit intensities on transport and distribution of C-14-assimilates in micropropagated apple plants. Li, TH,Li, SH,Wang, J,Yu, KS. 2003

[5]Topology and cellular localization of the small hydrophobic protein of avian metapneumovirus. Deng, Qiji,Weng, Yuejin,Li, Feng,Weng, Yuejin,Lu, Wuxun,Demers, Andrew,Wang, Dan,Li, Feng,Weng, Yuejin,Lu, Wuxun,Demers, Andrew,Wang, Dan,Li, Feng,Song, Minxun,Yu, Qingzhong. 2011

[6]Modeling of waterflow and nitrate transport under surface drip fertigation. Li, J,Zhang, J,Rao, M. 2005

[7]Influence of a heavy rainfall event on the leaching of [C-14]isoproturon and its degradation products in outdoor lysimeters. Doerfler, Ulrike,Cao, Guoyin,Grundmann, Sabine,Schroll, Reiner.

[8]The surface-pore integrated effect of soil organic matter on retention and transport of pharmaceuticals and personal care products in soils. Qin, Qin,Chen, Xijuan,Zhuang, Jie,Qin, Qin,Zhuang, Jie.

[9]Effect of Localized Scorch on the Transport and Distribution of Exogenous Jasmonic Acid in Vicia faba. Liu, X,Zhang, SQ,Lou, CH,Yu, FY.

[10]Studies on resistance characteristic and cDNA sequence conservation of transferrin from crucian carp, Carassius auratus. Long, Hua,Yu, Qi-Xing.

[11]Correlation between AS1 gene expression and seed protein contents in different soybean (Glycine max [L.] merr.) cultivars. Wan, TF,Shao, GH,Shan, XC,Zeng, NY,Lam, HM.

[12]OsPTF1, a novel transcription factor involved in tolerance to phosphate starvation in rice. Yi, KK,Wu, ZC,Zhou, J,Du, LM,Guo, LB,Wu, YR,Wu, P.

[13]Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants. Ruan, Jianyun,Gerendas, Joska,Hardter, Rolf,Sattelmacher, Burkhard.

[14]A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development. Chen, Qian,Liu, Yang,Maere, Steven,Van Isterdael, Gert,Xuan, Wei,Vassileva, Valya,Kitakura, Saeko,Wabnik, Krzysztof,Friml, Jiri,Beeckman, Tom,Vanneste, Steffen,Chen, Qian,Liu, Yang,Maere, Steven,Van Isterdael, Gert,Xuan, Wei,Vassileva, Valya,Kitakura, Saeko,Wabnik, Krzysztof,Friml, Jiri,Beeckman, Tom,Vanneste, Steffen,Chen, Qian,Li, Chuanyou,Liu, Yang,Lee, Eunkyoung,Lucas, Jessica,Sack, Fred,Xie, Zidian,Grotewold, Erich,Xie, Zidian,Grotewold, Erich,Vassileva, Valya,Kitakura, Saeko,Marhavy, Peter,Wabnik, Krzysztof,Benkova, Eva,Friml, Jiri,Marhavy, Peter,Geldner, Niko,Le, Jie,Fukaki, Hidehiro.

[15]Transport of a Novel Angiotensin-I-Converting Enzyme Inhibitory Peptide Ala-His-Leu-Leu Across Human Intestinal Epithelial Caco-2 Cells. Li, Ying,Zhao, Jiangtao,Liu, Xiaoli,Xia, Xiudong,Wang, Ying,Zhou, Jianzhong.

[16]Net cadmium flux and gene expression in relation to differences in cadmium accumulation and translocation in four apple rootstocks. Zhou, Jiangtao,Wan, Huixue,Qin, Sijun,He, Jiali,Lyu, Deguo,Zhou, Jiangtao,Wan, Huixue,Qin, Sijun,He, Jiali,Lyu, Deguo,Li, Huifeng.

[17]Inventory and general analysis of the ATP-binding cassette (ABC) gene superfamily in maize (Zea mays L.). Pang, Kaiyuan,Li, Yanjiao,Liu, Menghan,Meng, Zhaodong,Yu, Yanli,Pang, Kaiyuan.

[18]Host Cellular Protein TRAPPC6A Delta Interacts with Influenza A Virus M2 Protein and Regulates Viral Propagation by Modulating M2 Trafficking. Zhu, Pengyang,Liang, Libin,Shao, Xinyuan,Luo, Weiyu,Jiang, Shuitao,Zhao, Qingqing,Sun, Nan,Zhao, Yuhui,Li, Junping,Wang, Jinguang,Zhou, Yuan,Zhang, Jie,Wang, Guangwen,Jiang, Li,Chen, Hualan,Li, Chengjun.

[19]Ionomic and transcriptomic analysis provides new insight into the distribution and transport of cadmium and arsenic in rice. Feng, Xuemin,Han, Lei,Liu, Yan,Zhang, Yajing,Wang, Ruigang,Guo, Junkang,Feng, Renwei,Xu, Yingming,Ding, Yongzhen,Zhang, Guilong,Feng, Xuemin,Han, Lei,Liu, Yan,Zhang, Yajing,Wang, Ruigang,Guo, Junkang,Feng, Renwei,Xu, Yingming,Ding, Yongzhen,Zhang, Guilong,Chao, Daiyin,Huang, Biyan.

[20]Comparative responses to silicon and selenium in relation to cadmium uptake, compartmentation in roots, and xylem transport in flowering Chinese cabbage (Brassica campestris L. ssp chinensis var. utilis) under cadmium stress. Wu, Zhichao,Wang, Fuhua,Liu, Shuai,Du, Yingqiong,Li, Furong,Du, Ruiying,Wen, Dian,Zhao, Jie,Wu, Zhichao,Wang, Fuhua,Liu, Shuai,Du, Yingqiong,Li, Furong,Du, Ruiying,Wen, Dian,Zhao, Jie,Wu, Zhichao,Wang, Fuhua,Liu, Shuai,Du, Yingqiong,Li, Furong,Du, Ruiying,Wen, Dian,Zhao, Jie,Liu, Shuai.

作者其他论文 更多>>