Kinetics of manganese transport and gene expressions of manganese transport carriers in Caco-2 cell monolayers
文献类型: 外文期刊
第一作者: Li, Xiaoli
作者: Li, Xiaoli;Xie, Jingjing;Lu, Lin;Zhang, Liyang;Zhang, Lingyan;Luo, Xugang;Li, Xiaoli;Zou, Yaxue;Wang, Qiuyue;Li, Sufen
作者机构:
关键词: Caco-2 cell;Divalent metal transporter 1;Ferroportin;Manganese;Transport
期刊名称:BIOMETALS ( 影响因子:2.949; 五年影响因子:2.978 )
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收录情况: SCI
摘要: Two experiments were conducted to investigate the kinetics of manganese (Mn) transport in Caco-2 cell monolayers and the gene expressions of Mn transport carriers in apical (AP) and basolateral (BL) membranes. In experiment 1, the cells were treated with the medium containing 146 μmol/L of Mn (MnSO_4·H_2O). Both the uptake and transport of Mn from AP-BL or from BL-AP at different time-points were assessed to determine the optimal time for kinetics of Mn transport. The transport of Mn increased linearly with higher efficiency values in AP-BL than in BL-AP direction, however, the uptake of Mn revealed an asymptotic pattern within 120 min. In experiment 2, the kinetics of Mn transport in AP-BL was determined with media containing Mn concentrations from 0 to 2,500 μmol/L at 40 and 120 min, respectively, and mRNA levels of divalent metal transporter 1 (DMT1) and ferroportin (FPN1) were determined in Caco-2 cells treated with the medium containing 0 or 800 μmol/L of Mn for 120 min. The kinetics of Mn transport showed a carrier-mediated process when Mn concentrations were lower than 1,000 μmol/L and a linear increment when Mn concentrations exceeded 1,000 μmol/L at either 40 or 120 min. Mn treatment decreased (P < 0.01) DMT1 mRNA level and increased (P < 0.01) FPN1 mRNA level. The results from the present study suggested that Mn transport in AP-BL fit both carrier-mediated saturable and non-saturable diffusion processes, and Mn transport carriers DMT1 and FPN1 mediate the apical uptake and basolateral exit of Mn in Caco-2 cells.
分类号: Q
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