Identification of a conserved B-cell epitope in the equine arteritis virus (EAV) N protein using the pepscan technique

文献类型： 外文期刊

第一作者： Zhao, Shihua

作者： Zhao, Shihua;Qi, Ting;Guo, Wei;Lu, Gang;Xiang, Wenhua

作者机构：

关键词： EAV;Epitope;N Protein;Pepscan technique

期刊名称：VIRUS GENES （ 影响因子：2.332； 五年影响因子：2.0 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The nucleocapsid (N) gene of equine arteritis virus (EAV) is highly conserved between isolates, and the N protein is an important antigen that induces immunity when horses are infected with EAV. This study describes the identification of a linear B-cell epitope on the N protein using the pepscan technique with a monoclonal antibody (mAb) 2B1 directed against the N protein. The N protein was divided into 11 overlapping peptides, each containing 16 amino acids associated with six overlapping amino acids. The fragments were expressed as MBP fusion proteins that were then used to probe the 2B1 mAb. The minimal epitope sequence was confirmed step-by-step using single amino acid residue deletion. One completely conserved linear epitope (38KPPAQP 43) was identified that matched with EAV-positive serum in Western blots, thereby revealing the importance of these six amino acids of the epitope for antibody-epitope binding activity. This finding not only contributes to our understanding of the antigenic structure of the N protein of EAV but also has potential for the development of diagnostic techniques.

分类号： R37

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