Preparation of cross-linked enzyme aggregates in water-in-oil emulsion: Application to trehalose synthase

文献类型： 外文期刊

第一作者： Chen, Ying

作者： Chen, Ying;Chen, Xiao-yun;Yang, Li-wei;Qi, Xin;Zhang, Jun;Xiao, Chen-peng;Zheng, Jian-feng;Li, Ming-chun

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关键词： Trehalose synthase;Spherical cross-linked enzyme aggregates;Water-in-oil emulsion

期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）

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收录情况： SCI

摘要： In this work, a novel method to prepare cross-linked enzyme aggregates (CLEA) in a water-in-oil emulsion was reported. Spherical CLEA of recombinant trehalose synthase (TreS) from Meiothermus rubber was obtained through the emulsion based process. By varying the experimental parameters, the optimum conditions for the preparation of spherical CLEA were determined. The optimum catalytic temperature and thermal stability of spherical CLEA were enhanced significantly compared with the free enzyme. Spherical CLEA was also more tolerant to metal ions and chemical denaturants. Optical and scanning electron microscopy analysis showed that instead of the amorphous clumping shape of conventionally prepared CLEA, spherical CLEA has a more structured ball-like appearance with a size of 20-60 μm in diameter that could be easily separated.

分类号： O64`Q55`O643

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