Establishment and characterization of a fibroblast line from Landrace

文献类型： 外文期刊

第一作者： Bai, Chunyu

作者： Bai, Chunyu;Jin, Dapeng;Guan, Weijun;Ma, Yuehui;Zhao, Qianjun;Bai, Chunyu;Guo, Yu;Li, Chunying

作者机构：

关键词： Biological characteristics;Cell bank;Fibroblast;Landrace

期刊名称：ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY （ 影响因子：1.314； 五年影响因子：1.048 ）

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收录情况： SCI

摘要： Up to 32 Landrace ear marginal tissue samples were used for establishing a fibroblast cell bank by the means of primary explantation and cryopreservation. Biological analysis suggested that the Population Doubling Time (PDT) of the cell line was approximately 24h. The diploid accounted for 97.2% of the whole population; isozyme analysis of Lactic Dehydrogenase (LDH) and Malic Dehydrogenase(MDH) disproved cross-contamination from other cell lines. The results of bacterium, fungus, virus and mycoplasma tests were all negative. The transfection rates of three fluorescent proteins were high, indicating that the exogenous genes could be effectively expressed in the cells. It had not only preserved the precious germplasm resource of the Landrace on the cell level but also provided valuable material for the researches of genomics, postgenomics, somatic cloning and so on.

分类号： R318

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